The development of genetically encoded dopamine sensors such as dLight has provided a new approach to measuring slow and fast dopamine dynamics both in brain slices and in vivo, possibly enabling dopamine measurements in areas like the dorsolateral striatum (DLS) where previously such recordings with fast-scan cyclic voltammetry (FSCV) were difficult. To test this, we first evaluated dLight photometry in mouse brain slices with simultaneous FSCV and found that both techniques yielded comparable results, but notable differences in responses to dopamine transporter inhibitors, including cocaine. We then used in vivo fiber photometry with dLight in mice to examine responses to cocaine in DLS. We also compared dopamine responses during Pavlovian conditioning across the striatum. We show that dopamine increases were readily detectable in DLS and describe transient dopamine kinetics, as well as slowly developing signals during conditioning. Overall, our findings indicate that dLight photometry is well suited to measuring dopamine dynamics in DLS.

dLight 等基因编码多巴胺传感器的发展提供了一种测量脑切片和体内慢速和快速多巴胺动态的新方法，可能使背外侧纹状体 (DLS) 等区域的多巴胺测量成为可能，而这些区域以前是通过快速记录进行多巴胺测量的。扫描循环伏安法（FSCV）很困难。为了测试这一点，我们首先使用同步 FSCV 评估小鼠脑切片中的 dLight 光度测定，发现两种技术产生的结果相当，但对多巴胺转运蛋白抑制剂（包括可卡因）的反应存在显着差异。然后，我们在小鼠体内使用 dLight 进行体内光纤光度测定，以检查 DLS 中对可卡因的反应。我们还比较了纹状体巴甫洛夫调节过程中的多巴胺反应。我们表明，在 DLS 中很容易检测到多巴胺的增加，并描述了瞬态多巴胺动力学，以及调节过程中缓慢产生的信号。总体而言，我们的研究结果表明 dLight 光度测定法非常适合测量 DLS 中的多巴胺动态。