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Ustilaginoidea virens-secreted effector Uv1809 suppresses rice immunity by enhancing OsSRT2-mediated histone deacetylation
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2023-09-16 , DOI: 10.1111/pbi.14174 Xiaoyang Chen 1 , Chen Liu 1 , Hailin Wang 1 , Qi Liu 1 , Yaping Yue 2 , Yuhang Duan 3 , Zhaoyun Wang 1 , Lu Zheng 3 , Xiaolin Chen 4 , Yaohui Wang 1, 5 , Junbin Huang 3 , Qiutao Xu 2 , Yuemin Pan 1
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2023-09-16 , DOI: 10.1111/pbi.14174 Xiaoyang Chen 1 , Chen Liu 1 , Hailin Wang 1 , Qi Liu 1 , Yaping Yue 2 , Yuhang Duan 3 , Zhaoyun Wang 1 , Lu Zheng 3 , Xiaolin Chen 4 , Yaohui Wang 1, 5 , Junbin Huang 3 , Qiutao Xu 2 , Yuemin Pan 1
Affiliation
Rice false smut caused by Ustilaginoidea virens is a devastating rice (Oryza sativa) disease worldwide. However, the molecular mechanisms underlying U. virens–rice interactions are largely unknown. In this study, we identified a secreted protein, Uv1809, as a key virulence factor. Heterologous expression of Uv1809 in rice enhanced susceptibility to rice false smut and bacterial blight. Host-induced gene silencing of Uv1809 in rice enhanced resistance to U. virens, suggesting that Uv1809 inhibits rice immunity and promotes infection by U. virens. Uv1809 suppresses rice immunity by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation, thereby reducing H4K5ac and H4K8ac levels and interfering with the transcriptional activation of defence genes. CRISPR-Cas9 edited ossrt2 mutants showed no adverse effects in terms of growth and yield but displayed broad-spectrum resistance to rice pathogens, revealing a potentially valuable genetic resource for breeding disease resistance. Our study provides insight into defence mechanisms against plant pathogens that inactivate plant immunity at the epigenetic level.
中文翻译:
黑粉菌分泌的效应子 Uv1809 通过增强 OsSRT2 介导的组蛋白脱乙酰化来抑制水稻免疫
由Ustilaginoidea virens引起的水稻稻曲病是世界范围内一种毁灭性的水稻病害。然而, U 的分子机制。绿藻与水稻的相互作用在很大程度上是未知的。在这项研究中,我们确定了一种分泌蛋白 Uv1809,它是一个关键的毒力因子。水稻中Uv1809的异源表达增强了对稻曲病和白叶枯病的易感性。水稻中宿主诱导的Uv1809基因沉默增强了对U的抗性。绿菌,表明Uv1809抑制水稻免疫力并促进绿菌感染。维伦斯。 Uv1809通过靶向和增强水稻组蛋白脱乙酰酶OsSRT2介导的组蛋白脱乙酰化来抑制水稻免疫,从而降低H4K5ac和H4K8ac水平并干扰防御基因的转录激活。 CRISPR-Cas9编辑的ossrt2突变体在生长和产量方面没有表现出不利影响,但对水稻病原体表现出广谱抗性,揭示了育种抗病性的潜在宝贵遗传资源。我们的研究深入了解了植物病原体的防御机制,这些病原体在表观遗传水平上使植物免疫失活。
更新日期:2023-09-16
中文翻译:
黑粉菌分泌的效应子 Uv1809 通过增强 OsSRT2 介导的组蛋白脱乙酰化来抑制水稻免疫
由Ustilaginoidea virens引起的水稻稻曲病是世界范围内一种毁灭性的水稻病害。然而, U 的分子机制。绿藻与水稻的相互作用在很大程度上是未知的。在这项研究中,我们确定了一种分泌蛋白 Uv1809,它是一个关键的毒力因子。水稻中Uv1809的异源表达增强了对稻曲病和白叶枯病的易感性。水稻中宿主诱导的Uv1809基因沉默增强了对U的抗性。绿菌,表明Uv1809抑制水稻免疫力并促进绿菌感染。维伦斯。 Uv1809通过靶向和增强水稻组蛋白脱乙酰酶OsSRT2介导的组蛋白脱乙酰化来抑制水稻免疫,从而降低H4K5ac和H4K8ac水平并干扰防御基因的转录激活。 CRISPR-Cas9编辑的ossrt2突变体在生长和产量方面没有表现出不利影响,但对水稻病原体表现出广谱抗性,揭示了育种抗病性的潜在宝贵遗传资源。我们的研究深入了解了植物病原体的防御机制,这些病原体在表观遗传水平上使植物免疫失活。