This work proposes a novel zero discharge, carbon-neutral bioprocess for the simultaneous production of algal biodiesel and renewable hydrogen. This innovative bi-fuel production process (BiFPP) consists of five units connected in series for biodiesel production, biodiesel purification, glycerol separation, glycerol steam reforming and hydrogen purification. To assess the techno-economic feasibility, the genetic algorithm based multi-objective optimization strategy is developed to minimize the total annual cost and CO₂ emission level, and maximize the fuel purity. The optimal BiFPP scheme yields 97.3 mol % biodiesel and 98.8 mol % hydrogen, and thereby meets the standard specifications, ASTM D6751 and EN 14214. A heat exchanger network is designed with thermal coupling to secure the energy savings of 79.5% (heating load) and 50% (cooling load), leading to 93% reduction in utility cost. To achieve carbon neutrality, a CO₂ sink for algae cultivation is finally proposed to integrate with this industrial-scale BiFPP process that overall yields a novel zero discharge bi-fuel production technology.

这项工作提出了一种新颖的零排放、碳中性生物工艺，用于同时生产藻类生物柴油和可再生氢气。这种创新的双燃料生产工艺（BiFPP）由五个串联的单元组成，用于生物柴油生产、生物柴油纯化、甘油分离、甘油蒸汽重整和氢气纯化。为了评估技术经济可行性，开发了基于遗传算法的多目标优化策略，以最小化年度总成本和CO ₂排放水平，并最大化燃料纯度。最佳 BiFPP 方案可产生 97.3 mol% 生物柴油和 98.8 mol% 氢气，从而满足标准规范 ASTM D6751 和 EN 14214。网络采用热耦合设计，确保节能 79.5%（热负荷）和 50%（冷负荷），从而使公用事业成本降低 93%。为了实现碳中和，最终提出了一种用于藻类培养的CO _{2汇，与这种工业规模的BiFPP工艺相结合，总体上产生了一种新型的零排放双燃料生产技术。}