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Study on Mechanisms of Resistance to SDHI Fungicide Pydiflumetofen in Fusarium fujikuroi
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-09-20 , DOI: 10.1021/acs.jafc.3c03678 Yu Liu 1 , Yang Sun 1, 2, 3, 4 , Yang Bai 1 , Xin Cheng 1 , Hui Li 1 , Xing Chen 1, 2, 3, 4 , Yu Chen 1, 2, 3, 4
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-09-20 , DOI: 10.1021/acs.jafc.3c03678 Yu Liu 1 , Yang Sun 1, 2, 3, 4 , Yang Bai 1 , Xin Cheng 1 , Hui Li 1 , Xing Chen 1, 2, 3, 4 , Yu Chen 1, 2, 3, 4
Affiliation
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Rice bakaenii disease (RBD) is a widespread and devastating disease mainly caused by Fusarium fujikuroi. Pydiflumetofen (Pyd) is a novel succinate dehydrogenase inhibitor (SDHI) with strong inhibitory activity against F. fujikuroi, but the mechanism of resistance to Pyd has not been well studied for this pathogen. Through fungicide adaption, a total of 12 Pyd-resistant mutants were obtained and the resistance level could be divided into three categories of high resistance (HR), moderate resistance (MR), and low resistance (LR) with resistance factors (RF) of 184.04–672.90, 12.63–42.49, and <10, respectively. Seven genotypes of point mutations in FfSdh genes (FfSdhBH248L, FfSdhBH248D, FfSdhBH248Y, FfSdhC2A83V, FfSdhC2H144Y, FfSdhDS106F, and FfSdhDE166K) were found in these mutants, among which genotype FfSdhBH248L and FfSdhC2A83V mutants showed HR, genotype FfSdhBH248D, FfSdhBH248Y, FfSdhC2H144Y, and FfSdhDE166K mutants showed MR, and genotype FfSdhDS106F mutants showed LR. Moreover, all the substitutions of amino acid point mutations including FfSdhBH248L/D/Y, FfSdhC2A83V,H144Y, and FfSdhDS106F,E166K conferring resistance to Pyd in F. fujikuroi were verified by protoplast transformation. Additionally, a positive cross-resistance was detected between Pyd and another SDHI fungicide penflufen, while no cross-resistance was detected between Pyd and phenamacril, prochloraz, azoxystrobin, carbendazim, or fludioxonil. Although pathogenicity of the mutants was increased compared with that of the wild-type parental strains, the mycelial growth rate and spore production levels of the resistant mutants were significantly decreased (P < 0.05), indicating significant fitness cost of resistance to Pyd in F. fujikuroi. Taken together, the risk of resistance to Pyd in F. fujikuroi might be moderate, and appropriate precautions against resistance development in natural populations should be taken into account when Pyd is used for the control of RBD.
中文翻译：
藤黑镰刀菌对SDHI杀菌剂吡氟菌酯的抗性机制研究
水稻巴卡尼病（RBD）是一种广泛传播的毁灭性病害，主要由藤黑镰刀菌引起。Pydiflumetofen (Pyd) 是一种新型琥珀酸脱氢酶抑制剂 (SDHI)，对藤黑镰刀菌具有较强的抑制活性，但对该病原菌的耐药机制尚未得到充分研究。通过杀菌剂适应，共获得12个Pyd抗性突变体，抗性水平可分为高抗性（HR）、中抗性（MR）和低抗性（LR）三类，抗性因子（RF）为分别为 184.04–672.90、12.63–42.49 和 <10。在这些突变体中发现了7种FfSdh基因点突变基因型（FfSdhB H248L、FfSdhB H248D、FfSdhB H248Y、FfSdhC 2 A83V、FfSdhC 2 H144Y、FfSdhD S106F和FfSdhD E166K ），其中基因型为FfSdhB H2 48L和 FfSdhC 2 A83V突变体显示HR、基因型FfSdhB H248D、FfSdhB H248Y、FfSdhC 2 H144Y和FfSdhD E166K突变体显示MR，基因型FfSdhD S106F突变体显示LR。此外，通过原生质体转化验证了FfSdhB H248L/D/Y、FfSdhC 2 A83V、H144Y和FfSdhD S106F、E166K等氨基酸点突变的所有取代，这些突变赋予F. fujikuroi对Pyd的抗性。此外，Pyd 与另一种 SDHI 杀菌剂戊氟芬之间检测到正交叉耐药性，而 Pyd 与非那丙烯利、咪鲜胺、嘧菌酯、多菌灵或咯菌腈之间未检测到交叉耐药性。尽管与野生型亲本菌株相比，突变体的致病力有所增加，但抗性突变体的菌丝生长速度和孢子产生水平显着降低（P < 0.05），表明F.藤黑井。总而言之，F. fujikuroi对 Pyd 产生抗性的风险可能是中等的，当 Pyd 用于控制 RBD 时，应考虑采取适当的预防措施来防止自然种群中产生抗性。
更新日期：2023-09-20
中文翻译：
藤黑镰刀菌对SDHI杀菌剂吡氟菌酯的抗性机制研究
水稻巴卡尼病（RBD）是一种广泛传播的毁灭性病害，主要由藤黑镰刀菌引起。Pydiflumetofen (Pyd) 是一种新型琥珀酸脱氢酶抑制剂 (SDHI)，对藤黑镰刀菌具有较强的抑制活性，但对该病原菌的耐药机制尚未得到充分研究。通过杀菌剂适应，共获得12个Pyd抗性突变体，抗性水平可分为高抗性（HR）、中抗性（MR）和低抗性（LR）三类，抗性因子（RF）为分别为 184.04–672.90、12.63–42.49 和 <10。在这些突变体中发现了7种FfSdh基因点突变基因型（FfSdhB H248L、FfSdhB H248D、FfSdhB H248Y、FfSdhC 2 A83V、FfSdhC 2 H144Y、FfSdhD S106F和FfSdhD E166K ），其中基因型为FfSdhB H2 48L和 FfSdhC 2 A83V突变体显示HR、基因型FfSdhB H248D、FfSdhB H248Y、FfSdhC 2 H144Y和FfSdhD E166K突变体显示MR，基因型FfSdhD S106F突变体显示LR。此外，通过原生质体转化验证了FfSdhB H248L/D/Y、FfSdhC 2 A83V、H144Y和FfSdhD S106F、E166K等氨基酸点突变的所有取代，这些突变赋予F. fujikuroi对Pyd的抗性。此外，Pyd 与另一种 SDHI 杀菌剂戊氟芬之间检测到正交叉耐药性，而 Pyd 与非那丙烯利、咪鲜胺、嘧菌酯、多菌灵或咯菌腈之间未检测到交叉耐药性。尽管与野生型亲本菌株相比，突变体的致病力有所增加，但抗性突变体的菌丝生长速度和孢子产生水平显着降低（P < 0.05），表明F.藤黑井。总而言之，F. fujikuroi对 Pyd 产生抗性的风险可能是中等的，当 Pyd 用于控制 RBD 时，应考虑采取适当的预防措施来防止自然种群中产生抗性。
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