<br>一种基于发光 MOF 的非酶探针，用于体液中尿酸的比色/光热/荧光三重模式测定,Talanta

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一种基于发光 MOF 的非酶探针，用于体液中尿酸的比色/光热/荧光三重模式测定
Talanta ( IF 5.6 ) Pub Date : 2023-09-15 , DOI: 10.1016/j.talanta.2023.125201
Jianping Guan ₁ , Meng Wang ₁ , Yu Xiong ₁ , Qi Liu ₁ , Xiaoqing Chen ₂

Affiliation

监测体液中尿酸（UA）水平对相关疾病的临床诊断和治疗具有重要意义。在此，提出了一种基于新型纳米复合 R6G@Fe-MOF 的非酶探针，为视觉、灵敏和方便的 UA 检测提供比率荧光、比色和光热三重读数信号。发现原位封装的 R6G@Fe-MOF 的框架结构在加入 UA 后分解，导致 Fe3+ 还原为 Fe2+。这种减少将导致 430 nm 处的荧光发射（FL）迅速增加。同时，由于 UA 和 R6G@Fe-MOF 之间的内部滤光效应（IFE），573 nm 处的 FL 将显着降低。此外，生成的 Fe2+ 与铁氰化钾（K3 [Fe（CN）6]）反应可原位生成具有出色颜色和光热特性的普鲁士蓝（PBNP），从而易于比色和光热信号读数。比色、荧光和光热检测的检测限（LOD）较低，分别为 1.68 μM、0.236 μM 和 1.32 μM。最终，它被成功用于测定尿液、血清和唾液中的 UA，产生了令人满意的结果。构建的 R6G@Fe-MOF 传感器提供了简单、灵敏和准确的 UA 测定方法，可以进行定制以满足各种应用的需求，也为设计和开发适用于各种用途的多功能传感器提供了新的视角。

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A luminescent MOF-based nonenzymatic probe for colorimetric/photothermal/fluorescence triple-mode assay of uric acid in body fluids

Monitoring the levels of uric acid (UA) in body fluids is of great significance in the clinical diagnosis and therapy of related diseases. Herein, a novel nanocomposite R6G@Fe-MOF based nonenzymatic probe is presented to provide a ratiometric fluorescent, colorimetric, and photothermal triple read-out signal for the visual, sensitive, and convenient assay of UA. The framework structure of the in situ encapsulated R6G@Fe-MOF is found to decompose upon the addition of UA, resulting in the reduction of Fe3+ to Fe2+. This reduction will lead to a rapid increase in fluorescence emission (FL) at 430 nm. Simultaneously, the FL at 573 nm will decrease remarkably due to the inner filter effect (IFE) between UA and R6G@Fe-MOF. Furthermore, the reaction of the generated Fe2+ with potassium ferricyanide (K3 [Fe(CN)6]) can in situ generate Prussian blue (PBNPs) with outstanding color and photothermal properties, which allow for easy colorimetric and photothermal signal readout. The detection limits (LOD) for the colorimetric, fluorometric and photothermal detection are low at 1.68 μM, 0.236 μM, and 1.32 μM respectively. Ultimately, it is successfully employed to determine UA in urine, serum, and saliva, yielding satisfactory results. The constructed R6G@Fe-MOF sensor provides a simple, sensitive, and accurate determination of UA that can be tailored to meet the needs of various applications, and also provides new perspectives for the design and development of versatile sensors for diverse uses.

更新日期：2023-09-15

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