Multi-omics analysis reveals the mechanism of action of ophiopogonin D against pulmonary fibrosis,Phytomedicine

当前位置： X-MOL 学术 › Phytomedicine › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Multi-omics analysis reveals the mechanism of action of ophiopogonin D against pulmonary fibrosis
Phytomedicine ( IF 6.7 ) Pub Date : 2023-09-14 , DOI: 10.1016/j.phymed.2023.155078
Shengchuan Bao ₁ , Ting Chen ₁ , Juan Chen ₁ , Jiaxiang Zhang ₁ , Guangjian Zhang ₂ , Yi Hui ₁ , Jingtao Li ₃ , Shuguang Yan ₁

Affiliation

Background

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with limited therapeutic strategies. Therefore, there is an urgent need to search for safe and effective drugs to treat this condition. Ophiopogonin D (OP-D), a steroidal saponin compound extracted from ophiopogon, possesses various pharmacological properties, including anti-inflammatory, antioxidant, and antitumor effects. However, the potential pharmacological effect of OP-D on pulmonary fibrosis remains unknown.

Purpose

The aim of this study was to investigate whether OP-D can improve pulmonary fibrosis and to explore its mechanism of action.

Methods

The effect of OP-D on pulmonary fibrosis was investigated in vitro and in vivo using a mouse model of IPF induced by bleomycin and an in vitro model of human embryonic lung fibroblasts induced by transforming growth factor-β1 (TGF-β1). The mechanism of action of OP-D was determined using multi-omics techniques and bioinformatics.

Results

OP-D attenuated epithelial-mesenchymal transition and excessive deposition of extracellular matrix in the lungs, promoted the apoptosis of lung fibroblasts, and blocked the differentiation of lung fibroblasts into myofibroblasts. The multi-omics techniques and bioinformatics analysis revealed that OP-D blocked the AKT/GSK3β pathway, and the combination of a PI3K/AKT inhibitor and OP-D was effective in alleviating pulmonary fibrosis.

Conclusion

This study demonstrated for the first time that OP-D can reduce lung inflammation and fibrosis. OP-D is thus a potential new drug for the prevention and treatment of pulmonary fibrosis.

中文翻译：