Reconfigurable modular microfluidics presents an opportunity for flexibly constructing prototypes of advanced microfluidic systems. Nevertheless, the strategy of directly integrating modules cannot easily fulfill the requirements of common applications, e.g., the incorporation of materials with biochemical compatibility and optical transparency and the execution of small batch production of disposable chips for laboratory trials and initial tests. Here, we propose a manufacturing scheme inspired by the movable type printing technique to realize 3D free-assembly modular microfluidics. Double-layer 3D microfluidic structures can be produced by replicating the assembled molds. A library of modularized molds is presented for flow control, droplet generation and manipulation and cell trapping and coculture. In addition, a variety of modularized attachments, including valves, light sources and microscopic cameras, have been developed with the capability to be mounted onto chips on demand. Microfluidic systems, including those for concentration gradient generation, droplet-based microfluidics, cell trapping and drug screening, are demonstrated. This scheme enables rapid prototyping of microfluidic systems and construction of on-chip research platforms, with the intent of achieving high efficiency of proof-of-concept tests and small batch manufacturing.

可重构模块化微流体为灵活构建先进微流体系统原型提供了机会。然而，直接集成模块的策略并不能轻易满足常见应用的要求，例如结合具有生化相容性和光学透明性的材料以及用于实验室试验和初始测试的一次性芯片的小批量生产。在这里，我们提出了一种受活字印刷技术启发的制造方案，以实现3D自由组装模块化微流控。双层 3D 微流体结构可以通过复制组装的模具来生产。提供了一个模块化模具库，用于流量控制、液滴生成和操作以及细胞捕获和共培养。此外，还开发了各种模块化附件，包括阀门、光源和微型摄像机，能够根据需要安装到芯片上。演示了微流体系统，包括用于浓度梯度生成、基于液滴的微流体、细胞捕获和药物筛选的系统。该方案能够实现微流控系统的快速原型设计和片上研究平台的构建，旨在实现概念验证测试和小批量制造的高效率。