In this work, a two-stage electrostatic precipitator as the integration of a pre-charger and an electrostatic collector is proposed for airborne bio-aerosol sampling, where a pre-charger was applied for collection efficiencies enhancement and the following parallel-plate collector captured bio-aerosols with high biological viabilities. And the effects of operation parameters, including applied voltages, flow rates and collection area, on both the physical collection efficiency and biological viability are investigated. Specifically, the electric field strength of 4.3 kV/cm keeps the best balance between sampling efficiency and microorganism survival. For general designs and applications of present two-stage sampler, the modified ESP index and Pre-charger index are defined based on the charging characteristics of two typical bio-aerosols as Escherichia Coli and Candida albicans for predicting physical collection efficiency, biological viability and their ratio. A porotype with 2 × 10¹² s V² m⁻³ and 1.5 × 10¹⁴ s V² m⁻⁵ for ESP and Pre-charging Index, respectively, obtains collection performance 3–5 times higher than conventional AGI-30 sampler during an on-site sampling, proving its feasibility for bio-aerosol collection.

在这项工作中，提出了一种预充电器和静电收集器集成的两级静电除尘器用于空气生物气溶胶采样，其中预充电器用于提高收集效率，随后的平行板收集器捕获具有高生物活性的生物气溶胶。并研究了操作参数（包括施加电压、流速和收集面积）对物理收集效率和生物活力的影响。具体来说，电场强度4.3 kV/cm在采样效率和微生物存活之间保持了最佳平衡。对于现有两级采样器的一般设计和应用，根据大肠杆菌和白色念珠菌两种典型生物气溶胶的充电特性，定义了改进的ESP指数和预充电指数，用于预测物理收集效率、生物活力及其比率。^{2 × 10 12}  s V ² m ⁻³和 1.5 × 10 ¹⁴  s V ² m ⁻⁵的孔型在现场采样过程中，ESP和预充电指数分别获得了比传统AGI-30采样器高3-5倍的采集性能，证明了其用于生物气溶胶采集的可行性。