<br>长期暴露于可的松会诱导成年雌性斑马鱼（Danio rerio）的甲状腺内分泌紊乱和视网膜功能障碍,Science of the Total Environment

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长期暴露于可的松会诱导成年雌性斑马鱼（Danio rerio）的甲状腺内分泌紊乱和视网膜功能障碍
Science of the Total Environment ( IF 8.2 ) Pub Date : 2023-09-13 , DOI: 10.1016/j.scitotenv.2023.167022
Yuxin Geng ₁ , Hong Zou ₁ , Yanfang Guo ₁ , Manlin Huang ₁ , Yashi Wu ₁ , Liping Hou ₁

Affiliation

可的松在河流中含量很高，因为它具有广泛的医疗应用和自然分泌它的生物体的消除。作为一种类固醇激素，可的松被认为是一种新型内分泌干扰物。尽管最近主要报道了生殖内分泌系统的生态毒理学影响，但对鱼类甲状腺内分泌的了解仍然相对较少。在这里，成年雌性斑马鱼暴露于 0.0 （对照）、3.2、38.7 和 326.9 ng/L 的可的松 60 天。这项研究的证据来自鱼类的行为、激素水平、基因表达、组织学和形态学检查。结果显示，甲状腺发生 THs （甲状腺激素）水平破坏和病理组织学变化，这可能是下丘脑-垂体-甲状腺轴基因表达变化的原因。具体来说，T4（甲状腺素）向 T3（三碘甲状腺原氨酸）的更多转化导致血浆中的 TSH（促甲状腺激素）水平升高。严重的甲状腺组织损伤主要发生在暴露于 326.9 ng/L 可的松的斑马鱼中。同时，与 THs 趋势一致，鱼类的运动活动表现出更多的焦虑和兴奋，GABA （γ – 氨基丁酸）合成途径基因的部分阻断可能是潜在机制的解释。可的松影响视觉周期中的基因表达，昼夜节律网络也提示甲状腺内分泌紊乱、视网膜功能障碍和斑马鱼异常行为之间的相互作用。综上所述，这些发现表明长期接触可的松会对成年雌性斑马鱼产生各种不良反应，这可能有助于我们更好地了解可的松对野外鱼类的风险。

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Chronic exposure to cortisone induces thyroid endocrine disruption and retinal dysfunction in adult female zebrafish (Danio rerio)

Cortisone has a large content in rivers because of its wide range of medical applications and elimination by organisms that naturally secrete it. As a steroid hormone, cortisone is recognized as a novel endocrine disruptor. Although ecotoxicological effects of the reproductive endocrine system have mainly been reported recently, thyroid endocrine in fish remains relatively less understood. Here, adult female zebrafish were exposed to cortisone at 0.0 (control), 3.2, 38.7, and 326.9 ng/L for 60 days. Evidence in this study came from fish behavior, hormone levels, gene expression, histological and morphological examinations. The results showed that THs (thyroid hormone) level disruption and pathohistological changes occurred in the thyroid gland, which may account for the gene expression changes in the hypothalamus-pituitary-thyroid gland axis. Specifically, more conversion of T4 (thyroxine) to T3 (triiodothyronine) led to an increased TSH (thyroid stimulating hormone) level in plasma. Severe thyroid tissue damage mainly occurred in the zebrafish exposed to 326.9 ng/L of cortisone. Meanwhile, consistent with the THs trend, the fish locomotion activity displayed more anxiety and excitement, the partial blockage of GABA (γ – aminobutyric acid) synthetic pathway genes might be the explanation of the underlying mechanism. Cortisone affected the gene expressions in the visual cycle and the circadian rhythm network also suggested interactions between thyroid endocrine disruption, retinal dysfunction, and abnormal behaviors of zebrafish. In summary, these findings suggest chronic exposure to cortisone induced various adverse effects in adult female zebrafish, which may help us better understand the risk of cortisone to fish in the wild.

更新日期：2023-09-13

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