In this study, the ditriphenylamine Indole-BODIPY photosensitizer T₂BDP-lyso was synthesized for near-infrared photodynamic therapy and two-photon fluorescence imaging. The photosensitizer T₂BDP-lyso exhibits absorption above 700 nm and emission above 800 nm, respectively. Theoretical calculations show the energy gap from the excited state S₁ to the excited state T₂ is 0.14 eV, which indicated that the photosensitizer T₂BDP-lyso could reach the triplet state by intersystem crossing from the singlet state. Under NIR light, the singlet oxygen yield of photosensitizer T₂BDP-lyso was calculated to be 0.64 in CH₂Cl₂. The photosensitizer T₂BDP-lyso can effectively produce reactive oxygen species in A549 cells and zebrafish under 660 nm light for 5 min. The photosensitizer T₂BDP-lyso exhibited lower dark toxicity and higher phototoxicity (IC₅₀ = 1.49 μM), as well as lysosomal targeting ability (Pearson coefficient = 0.89). In the AO/EB double staining assay simulating photodynamic therapy at the cellular level, 3 μM of T₂BDP-lyso light for 10 min was effective in killing cancer cells. Moreover, the photosensitizer T₂BDP-lyso has a large two-photon absorption cross section at 1050 nm, which was calculated to be 138.7 GM in THF by Z-scan method, and two-photon fluorescence imaging was performed in zebrafish. The above results indicate the potential application of the photosensitizer T₂BDP-lyso in near-infrared photodynamic therapy and two-photon fluorescence imaging.

本研究合成了二三苯胺吲哚-BODIPY光敏剂T ₂ BDP-lyso，用于近红外光动力治疗和双光子荧光成像。光敏剂T ₂ BDP-lyso分别表现出700 nm以上的吸收和800 nm以上的发射。理论计算表明，激发态S ₁到激发态T ₂的能隙为0.14 eV，这表明光敏剂T ₂ BDP-lyso可以通过系间窜越从单线态达到三线态。在近红外光下，计算光敏剂T ₂ BDP-lyso在CH ₂ Cl ₂中的单线态氧产率为0.64 。光敏剂T ₂ BDP-lyso在660 nm光下5分钟可以有效地在A549细胞和斑马鱼中产生活性氧。光敏剂T ₂ BDP-lyso表现出较低的暗毒性和较高的光毒性（IC ₅₀  = 1.49 μM）以及溶酶体靶向能力（Pearson系数 = 0.89）。在模拟细胞水平光动力疗法的AO/EB双染色测定中，3μM的T ₂ BDP-lyso光持续10分钟可有效杀死癌细胞。此外，光敏剂T ₂ BDP-lyso在1050 nm处具有较大的双光子吸收截面，通过Z扫描方法计算出其在THF中的吸收截面为138.7 GM，并在斑马鱼中进行了双光子荧光成像。上述结果表明光敏剂T ₂ BDP-lyso在近红外光动力治疗和双光子荧光成像中的潜在应用。