Quality control for a traditional Chinese medicine, Millettia speciosa Champ, using ultra-high-performance liquid chromatography fingerprint, serum pharmacochemistry and network pharmacology,Analytical Methods

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Quality control for a traditional Chinese medicine, Millettia speciosa Champ, using ultra-high-performance liquid chromatography fingerprint, serum pharmacochemistry and network pharmacology
Analytical Methods ( IF 2.7 ) Pub Date : 2023-09-08 , DOI: 10.1039/d3ay01051a
Yunyuan Nong ₁ , Chi Zhang ₁ , Yue Guo _{1,

2,

3} , Yuelian Qin ₁ , Xinyu Zhong ₁ , Linlin Feng ₁ , Ziping Pan ₁ , Lijun Deng ₁ , Hongwei Guo _{1,

4} , Zhiheng Su _{1,

4,

5,

6}

Affiliation

Millettia speciosa (M. speciosa) Champ (MSC) is a healthy food type with medicinal and edible homology, which is now considered a clinically significant anti-rheumatoid arthritis medicine. However, there is currently no standardized or generally accepted research strategy by which we can assess M. speciosa. Thus, it is essential to develop novel theories, strategies and evaluation methods for the scientific quality control of M. speciosa. Herein, our use ultra-high-performance liquid chromatography (UPLC)-MS/MS analysis identified 12 common bioactive components absorbed into MSC serum. Next, network pharmacology analysis exhibited that 5 MSC components may be those active components in treating rheumatoid arthritis and may be considered potential quality markers. These 5 components were then quantified using a fast UPLC approach, based on the quality marker of measurability, showing that lenticin can be regarded as the MSC quality marker. The cumulative study findings, based on systematic assessment of chemical composition both in vivo and in vitro, and the potential efficacy of M. speciosa, provide a novel approach for M. speciosa quality control.

中文翻译：

采用超高效液相色谱指纹图谱、血清药物化学和网络药理学对中药鸡血藤进行质量控制

鸡血藤（M.speciosa）Champ（MSC）是一种药食同源的健康食品类型，目前被认为是具有临床意义的抗风湿性关节炎药物。然而，目前还没有标准化或普遍接受的研究策略来评估M. spiosa。因此，建立新的理论、策略和评价方法对大花木进行科学的质量控制十分必要。在此，我们使用超高效液相色谱 (UPLC)-MS/MS 分析鉴定了 MSC 血清中吸收的 12 种常见生物活性成分。接下来，网络药理学分析表明，5种MSC成分可能是治疗类风湿关节炎的活性成分，可以被认为是潜在的质量标志物。然后，基于可测量的质量标记，使用快速 UPLC 方法对这 5 种成分进行定量，表明菌毛素可被视为 MSC 质量标记。基于体内和体外化学成分的系统评估以及大花木的潜在功效，累积的研究结果为大花木的质量控制提供了一种新方法。

更新日期：2023-09-08

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