The Heuchera genus, a member of the Saxifragaceae family, encompasses a wide array of varieties and hybrids, serving both traditional medicinal and ornamental purposes. However, a significant knowledge gap persists in achieving efficient mass propagation of diverse Heuchera cultivars creating a substantial market void. To address this, our study focuses on expedited seedling regeneration by investigating leaf cutting and tissue culture techniques to offer novel insights to cultivators. Herein, we successfully rooted thirteen distinct cultivars from the Heuchera and Heucherella (Heuchera × Tiarella) genera through cutting. Moreover, in vitro culture experiments led to the successful induction of calli and shoots from petiole samples. Notably, variations in measured parameters were observed across cultivars in both cutting and tissue culture methodologies. When petiole explants were exposed to cytokinin 6-benzylaminopurine (BA) at concentrations of 0.5, 1.0, and 2.0 mg/L along with auxin α-naphthaleneacetic acid (NAA) at 0.5 mg/L, shoots were produced either directly or indirectly during the primary culture. Exposure to darkness and the application of 2,4-dichlorophenoxyacetic acid (2,4-D) did not promote shoot formation but were beneficial for callus stimulation. Interestingly, a negative correlation was observed between the ease of initiating cutting recovery and inducting tissue culture regeneration, suggesting that cultivars that easily regenerate through cutting might encounter difficulties during induction by tissue culture. In light of these findings, we devised a streamlined and effective protocol for rapid Heuchera propagation. This protocol involves micropropagation, directly acquiring adventitious shoots from primary cultures supplemented by cutting-based propagation methods.

Heuchera属属于虎耳草科，包含多种品种和杂交种，具有传统药用和观赏用途。然而，在实现不同Heuchera品种的有效大规模繁殖方面仍然存在巨大的知识差距，造成了巨大的市场空白。为了解决这个问题，我们的研究重点是通过研究切叶和组织培养技术来加速幼苗再生，为栽培者提供新的见解。在此，我们通过扦插成功地从Heuchera和Heucherella ( Heuchera × Tiarella ) 属中生根了 13 个不同的品种。此外，体外培养实验成功地从叶柄样品中诱导出愈伤组织和芽。值得注意的是，在切割和组织培养方法中都观察到了不同品种测量参数的变化。当叶柄外植体暴露于浓度为 0.5、1.0 和 2.0 mg/L 的细胞分裂素 6-苄氨基嘌呤 (BA) 以及浓度为 0.5 mg/L 的生长素 α-萘乙酸 (NAA) 时，在生长过程中直接或间接产生芽。主流文化。暴露于黑暗和施用2,4-二氯苯氧基乙酸(2,4-D)不会促进芽形成，但有利于愈伤组织刺激。有趣的是，在开始切割恢复的容易度和诱导组织培养再生之间观察到负相关，这表明通过切割容易再生的品种在组织培养诱导过程中可能会遇到困难。根据这些发现，我们设计了一种简化且有效的Heuchera快速繁殖方案。 该协议涉及微繁殖，直接从原代培养物中获取不定芽，并辅以基于切割的繁殖方法。