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Evaluating the Performance of the Astral Mass Analyzer for Quantitative Proteomics Using Data-Independent Acquisition
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2023-09-08 , DOI: 10.1021/acs.jproteome.3c00357 Lilian R Heil 1 , Eugen Damoc 2 , Tabiwang N Arrey 2 , Anna Pashkova 2 , Eduard Denisov 2 , Johannes Petzoldt 2 , Amelia C Peterson 2 , Chris Hsu 1 , Brian C Searle 3, 4 , Nicholas Shulman 1 , Michael Riffle 1 , Brian Connolly 1 , Brendan X MacLean 1 , Philip M Remes 5 , Michael W Senko 5 , Hamish I Stewart 2 , Christian Hock 2 , Alexander A Makarov 2 , Daniel Hermanson 5 , Vlad Zabrouskov 5 , Christine C Wu 1 , Michael J MacCoss 1
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2023-09-08 , DOI: 10.1021/acs.jproteome.3c00357 Lilian R Heil 1 , Eugen Damoc 2 , Tabiwang N Arrey 2 , Anna Pashkova 2 , Eduard Denisov 2 , Johannes Petzoldt 2 , Amelia C Peterson 2 , Chris Hsu 1 , Brian C Searle 3, 4 , Nicholas Shulman 1 , Michael Riffle 1 , Brian Connolly 1 , Brendan X MacLean 1 , Philip M Remes 5 , Michael W Senko 5 , Hamish I Stewart 2 , Christian Hock 2 , Alexander A Makarov 2 , Daniel Hermanson 5 , Vlad Zabrouskov 5 , Christine C Wu 1 , Michael J MacCoss 1
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We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data-independent acquisition, the Thermo Scientific Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Thermo Scientific Orbitrap mass spectrometers, which have long been the gold standard for high-resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer can produce high-quality quantitative measurements across a wide dynamic range. We also use a newly developed extracellular vesicle enrichment protocol to reach new depths of coverage in the plasma proteome, quantifying over 5000 plasma proteins in a 60 min gradient with the Orbitrap Astral mass spectrometer.
中文翻译：
使用数据独立采集评估 Astral Mass 分析仪的定量蛋白质组学性能
我们评估了新发布的非对称轨道无损（Astral）分析仪的定量性能。 Thermo Scientific Orbitrap Astral 质谱仪采用数据独立采集技术，每单位时间定量的肽数量是最先进的 Thermo Scientific Orbitrap 质谱仪的 5 倍，后者长期以来一直是高分辨率定量蛋白质组学的黄金标准。我们的结果表明 Orbitrap Astral 质谱仪可以在宽动态范围内产生高质量的定量测量。我们还使用新开发的细胞外囊泡富集方案来达到血浆蛋白质组的新覆盖深度，使用 Orbitrap Astral 质谱仪在 60 分钟梯度内定量超过 5000 种血浆蛋白质。
更新日期：2023-09-08
中文翻译：
使用数据独立采集评估 Astral Mass 分析仪的定量蛋白质组学性能
我们评估了新发布的非对称轨道无损（Astral）分析仪的定量性能。 Thermo Scientific Orbitrap Astral 质谱仪采用数据独立采集技术，每单位时间定量的肽数量是最先进的 Thermo Scientific Orbitrap 质谱仪的 5 倍，后者长期以来一直是高分辨率定量蛋白质组学的黄金标准。我们的结果表明 Orbitrap Astral 质谱仪可以在宽动态范围内产生高质量的定量测量。我们还使用新开发的细胞外囊泡富集方案来达到血浆蛋白质组的新覆盖深度，使用 Orbitrap Astral 质谱仪在 60 分钟梯度内定量超过 5000 种血浆蛋白质。
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