<br>Patescibacteria 的基因操作为微生物暗物质和附生生生活方式提供了机制见解,Cell

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Patescibacteria 的基因操作为微生物暗物质和附生生生活方式提供了机制见解
Cell ( IF 45.5 ) Pub Date : 2023-09-07 , DOI: 10.1016/j.cell.2023.08.017
Yaxi Wang ₁ , Larry A Gallagher ₁ , Pia A Andrade ₁ , Andi Liu ₁ , Ian R Humphreys ₂ , Serdar Turkarslan ₃ , Kevin J Cutler ₄ , Mario L Arrieta-Ortiz ₃ , Yaqiao Li ₅ , Matthew C Radey ₁ , Jeffrey S McLean ₆ , Qian Cong ₇ , David Baker ₈ , Nitin S Baliga ₃ , S Brook Peterson ₁ , Joseph D Mougous ₉

Affiliation

Department of Microbiology, University of Washington, Seattle, WA 98109, USA.
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.
Institute for Systems Biology, Seattle, WA 98109, USA.
Department of Physics, University of Washington, Seattle, WA 98195, USA.
Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Institute for Systems Biology, Seattle, WA 98109, USA.
Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Department of Periodontics, University of Washington, Seattle, WA 98195, USA.
Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98109, USA.
Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98109, USA; Microbial Interactions and Microbiome Center, University of Washington, Seattle, WA 98195, USA.

Patescibacteria，也称为候选门辐射（CPR），是一组多样化的细菌，在微生物暗物质中占不成比例的大部分。它为数不多的培养成员，主要属于糖菌门，在宿主放线菌门上以附生体的形式生长。由于缺乏合适的工具，这种生活方式的遗传基础和 Patescibacteira 的其他独特特征仍未得到探索。在这里，我们展示了糖杆菌表现出自然能力，我们利用这一特性进行基因操作。荧光蛋白标记的糖杆菌成像为伴随附生菌生长的现象提供了高时空分辨率，转座子插入测序（Tn-seq）全基因组筛选揭示了神秘的糖杆菌基因对其宿主生长的贡献。最后，我们利用宏基因组数据提供基于蛋白质结构的尖端生物信息学资源，以支持菌株 Southlakia epibionticum 及其相应的宿主 Actinomyces israelii，作为解锁附生生生活方式分子基础的模型系统。

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更新日期：2023-09-07

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