<br>负载 VEGF 的 PCL-mPEG 和负载 PDGF 的 PCL-壳聚糖双层血管移植物的物理化学、体外和体内评价,Journal of Biomedical Materials Research Part B: Applied Biomaterials

当前位置： X-MOL 学术 › J. Biomed. Mater. Res. Part B Appl. Biomater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

负载 VEGF 的 PCL-mPEG 和负载 PDGF 的 PCL-壳聚糖双层血管移植物的物理化学、体外和体内评价
Journal of Biomedical Materials Research Part B: Applied Biomaterials ( IF 3.2 ) Pub Date : 2023-09-07 , DOI: 10.1002/jbm.b.35325
Tamanna Sultana ₁ , Md Abdullah Al Fahad ₁ , Myeongki Park ₁ , Soon Ha Kwon ₂ , Byong-Taek Lee _{1,

3}

Affiliation

本研究试图评估新型双层小直径血管移植物的内皮化和平滑肌再生效率。制造了两种类型的层（PCL-mPEG-VEGF 和 PCL-壳聚糖-PDGF），以找出为管腔提供内皮化支持和为血管外层提供独特收缩功能的最佳层。通过基于氨解的表面修饰技术将血小板源性生长因子（PDGF）和壳聚糖固定到PCL表面。此外，将聚乙二醇甲醚（mPEG）和血管内皮生长因子（VEGF）直接与PCL混合。膜的形态分析确保平均纤维直径与天然细胞外基质的一致性。在体外研究中，PCL-mPEG-VEGF 与牛肺内皮细胞（CPAE）以及 PCL-壳聚糖-PDGF 与大鼠骨髓间充质干细胞（RBMSC）有良好的相互作用。在两层中都发现了受控的生长因子释放模式。此外，PCL-mPEG-VEGF 表现出 RBMSC 的内皮标志物表达特性。 PCL-壳聚糖-PDGF 膜显着确保了 SMC 标志物表达的上调。因此，PCL-mPEG-VEGF和PCL-壳聚糖-PDGF分别优选作为最终制备的管状杂化组织工程小直径血管移植物的内层和外层。最后，将双层血管移植物植入大鼠腹主动脉模型上2个月。提取的样品在内层中存在内皮细胞标记物（ICAM 1），在外层中存在平滑肌细胞标记物（αSMA），并且在两层中都观察到大量的胶原蛋白沉积。

"点击查看英文标题和摘要"

Physicochemical, in vitro and in vivo evaluation of VEGF loaded PCL-mPEG and PDGF loaded PCL-Chitosan dual layered vascular grafts

The present study has attempted to evaluate the endothelialization and smooth muscle regeneration efficiency of a novel dual-layer small-diameter vascular graft. Two types of layers (PCL-mPEG-VEGF and PCL-Chitosan-PDGF) were fabricated to find out the best layer giving endothelialization support for the lumen and unique contractile function for outer layer of blood vessels. Platelet-derived growth factor (PDGF) and chitosan were immobilized onto PCL surface by aminolysis-based surface modification technique. Besides, Poly (ethylene glycol) methyl ether (mPEG) and vascular endothelial growth factor (VEGF) were directly blended with PCL. Morphological analysis of membranes ensured consistency of average fibers diameter with native extracellular matrix. A favorable interaction of PCL-mPEG-VEGF with cow pulmonary endothelial cells (CPAEs) and PCL-Chitosan-PDGF with rat bone marrow mesenchymal stem cells (RBMSCs) was obtained during in vitro study. Controlled growth factor release patterns were found from both layers. Further, PCL-mPEG-VEGF exhibited endothelial markers expression properties from RBMSCs. Up-regulation of SMCs markers expression was significantly ensured by the PCL-Chitosan-PDGF membrane. Thus, PCL-mPEG-VEGF and PCL-Chitosan-PDGF were preferred as inner and outer layers respectively of a finally prepared tubular hybrid tissue engineered small diameter vascular graft. Finally, the dual-layer vascular graft was implanted onto a rat abdominal aorta model for 2 months. The extracted samples exhibited the presence of endothelial marker (ICAM 1) in the inner layer and smooth muscle cell marker (αSMA) in the outer layer as well as substantial amount of collagen deposition was observed in the both layers.

更新日期：2023-09-07

点击分享查看原文

点击收藏

阅读更多本刊新发论文