The liver is one of the most important organs responsible for detoxifying biomolecules and xenobiotics. Herein, we report the isolation, characterization, and hepatoprotective effect of the Boerhavia procumbens-derived eupalitin-3-O-β-D-galactopyranoside (EGP) compound. The structure of the EGP compound was deduced by using NMR spectroscopic techniques and mass spectrometry. The EGP hepatoprotective activities were evaluated with HepG2 cell viability and LDH assays in vitro, and CCl₄-induced toxicity was investigated in vivo in the rat model. Compared to the CCl₄-treated group, cells exposed to the EGP compound at 200 µg/ml showed increased cell viability (60.52 ± 1.22 %) and decreased LDH levels (23.81 ± 1.89 U/ml). The serum levels of SGPT, SGOT, ALP, and total bilirubin in the CCl4-treated group were substantially higher than those in the control group (64 ± 1.89 U/ml, 86 ± 1.47 U/ml, 252.6 ± 2.96 U/ml, and 5.45 ± 0.32 mg/dl, respectively). When compared to animals treated with CCl₄ alone, the EGP compound's in vivo hepatoprotective effect at 60 mg/kg with CCl₄ significantly (p < 0.01) decreased the levels of SGPT and SGOT (26 ± 1.34 U/ml and 42.92 ± 1.6 U/ml) respectively. Furthermore, our histological study showed a significant response in restoring and maintaining the normal morphological appearance of the liver. Thus, our results show that the EGP compound is a promising and novel lead molecule for better hepatotoxicity control and therapy.

肝脏是负责生物分子和异生物质解毒的最重要器官之一。在此，我们报道了Boerhavia procumbens衍生的 eupalitin-3-O - β -D-galactopyranoside (EGP)化合物的分离、表征和保肝作用。EGP化合物的结构是通过使用NMR波谱技术和质谱法推导出来的。通过体外HepG2细胞活力和LDH测定评估EGP的保肝活性，并在大鼠模型中研究CCl _4诱导的体内毒性。与CCl ₄处理组相比，暴露于200μg/ml EGP化合物的细胞显示出细胞活力增加（60.52±1.22%）和LDH水平降低（23.81±1.89U/ml）。CCl4治疗组血清SGPT、SGOT、ALP和总胆红素水平显着高于对照组（64±1.89 U/ml、86±1.47 U/ml、252.6±2.96 U/ml、和 5.45 ± 0.32 mg/dl，分别）。与单独用 CCl ₄治疗的动物相比，60 mg/kg 剂量的 EGP 化合物与 CCl _4的体内肝保护作用显着（p < 0.01）降低了 SGPT 和 SGOT 水平（26 ± 1.34 U/ml 和 42.92 ± 1.6 U） /毫升）分别。此外，我们的组织学研究显示在恢复和维持肝脏正常形态外观方面有显着的反应。因此，我们的结果表明，EGP 化合物是一种有前途的新型先导分子，可用于更好的肝毒性控制和治疗。