脑震荡小鼠模型体内与离体 MRI 指数的交叉比较分析,Brain Research

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脑震荡小鼠模型体内与离体 MRI 指数的交叉比较分析
Brain Research ( IF 2.7 ) Pub Date : 2023-09-04 , DOI: 10.1016/j.brainres.2023.148562
Xuan Vinh To ₁ , Nyoman D Kurniawan ₂ , Paul Cumming ₃ , Fatima A Nasrallah ₄

Affiliation

我们对结构磁共振成像 (MRI)、扩散张量成像 (DTI) 和神经突定向色散和密度成像 (NODDI) 测量以及脑震荡小鼠模型进行了横断面、病例匹配和成对比较，从而旨在建立活体大脑和固定后结构和扩散成像结果的一致性。我们将 28 只 3-4 个月大的雄性小鼠分配到假损伤和脑震荡 (CON) 组。 CON 小鼠在第 0 天受到单次震荡撞击，并在撞击后第 2 天 (n = 9) 或第 7 天 (n = 10) 接受 MRI，假对照组小鼠同样在第 2 天 (n = 5) 或 7 天接受成像（n = 4）。最终扫描后，我们立即收集了灌注固定的大脑，将其储存起来以供 6-12 个月后成像。然后我们比较了不同方法之间的结构成像、DTI 和 NODDI 结果。结构和 DTI/NODDI 研究结果在脑震荡对大脑结构完整性的影响方面的一致性明显较差。我们的研究结果要求在将获得的转化能力推断为生理测量时要谨慎。不同的发现可能反映了注视伪影和类淋巴系统变化的贡献。

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A cross-comparative analysis of in vivo versus ex vivo MRI indices in a mouse model of concussion

We present a cross-sectional, case-matched, and pair-wise comparison of structural magnetic resonance imaging (MRI), diffusion tensor imaging (DTI), and neurite orientation dispersion and density imaging (NODDI) measures and in a mouse model of concussion, thus aiming to establish the concordance of structural and diffusion imaging findings in living brain and after fixation. We allocated 28 male mice aged 3–4 months to sham injury and concussion (CON) groups. CON mice had received a single concussive impact on day 0 and underwent MRI at day 2 (n = 9) or 7 (n = 10) post-impact, and sham control mice likewise underwent imaging at day 2 (n = 5) or 7 (n = 4). Immediately after the final scanning, we collected the perfusion-fixed brains, which were stored for imaging 6–12 months later. We then compared the structural imaging, DTI, and NODDI results between different methods. to structural and DTI/NODDI findings were in notably poor agreement regarding the effects of concussion on structural integrity of the brain. Our findings call for caution in extrapolating translational capabilities obtained to physiological measurements . The divergent findings may reflect fixation artefacts and the contribution of the glymphatic system changes.

更新日期：2023-09-04

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