We report the development of a bifunctional near-infrared fluorescent probe (QZB) for selective sensing of bisulfite (HSO₃⁻) and hypochlorous acid (HOCl). The synergistic detection of HSO₃⁻ and HOCl was achieved via a C=C bond recognition site. In comparison with the red-fluorescence QZB, two different products with non-fluorescence and paleturquoise fluorescence were produced by the recognition of QZB towards HSO₃⁻ and HOCl respectively, which can realize effectively the dual-functional detection of HSO₃⁻ and HOCl. QZB features prominent preponderances of dual-function response, near-infrared emission, reliability at physiological pH, low cytotoxicity and high sensitivity to HSO₃⁻ and HOCl. The detection of HSO₃⁻ in actual food samples has been successfully achieved using QZB. Utilization of QZB-based test strip to semi-quantitatively detect HSO₃⁻ and HOCl in real-world water samples by the “naked-eye” colorimetry are then demonstrated. Simultaneously, the determination of HSO₃⁻ and HOCl in real-world water sample has been achieved by smartphone-based standard curves. Additionally, the applications of QZB for imaging HSO₃⁻ and HOCl in vivo are successfully demonstrated. Consequently, the successful development of QZB could be promising as an efficient tool for researching the role of HSO₃⁻/HOCl in the regulation of redox homeostasis regulation in vivo and complex signal transduction and for future food safety evaluation.

我们报告了一种双功能近红外荧光探针（QZB ）的开发，用于选择性传感亚硫酸氢盐（HSO ₃ ^- ）和次氯酸（HOCl）。_{HSO 3} ^-和HOCl的协同检测是通过C=C键识别位点实现的。与红色荧光QZB相比， QZB分别对HSO ₃ ^-和HOCl进行识别，产生了无荧光和浅绿松石色荧光两种不同的产物，可有效实现HSO ₃ ^-和HOCl的双功能检测。QZB具有双功能响应、近红外发射、生理pH下的可靠性、低细胞毒性以及对HSO ₃ ^-和HOCl的高敏感性等突出优势。使用QZB已成功实现了实际食品样品中HSO ₃ ⁻的检测。然后演示了利用基于QZB的测试条通过“肉眼”比色法半定量检测现实水样中的 HSO ₃ ^{-和 HOCl。}同时，通过基于智能手机的标准曲线实现了实际水样中HSO ₃ ^{−和 HOCl}的测定。此外， QZB在 HSO ₃ ^-和 HOCl体内成像的应用也得到了成功证明。因此， QZB的成功开发有望成为研究HSO ₃ ^{- /HOCl在}体内氧化还原稳态调节和复杂信号转导中的作用以及未来食品安全评估的有效工具。