A novel wastewater treatment plant process was constructed to overcome the challenge of simultaneous nitrate removal and phosphorus (P) recovery. The results revealed that the P and nitrate removal efficiency rose from 39.0 % and 48.4 % to 92.8 % and 93.6 % after 136 days of operation, and the total P content in the biofilm (TP_biofilm) rose from 15.8 mg/g SS to 57.8 mg/g SS. Moreover, the increase of TP_biofilm changed the metabolic mode of denitrifying polyphosphate accumulating organisms (DPAOs), increasing the P concentration of the enriched stream to 172.5 mg/L. Furthermore, the acid/alkaline fermentation led to the rupture of the cell membrane, which released poly-phosphate and ortho-phosphate of cell/EPS in DPAOs and released metal‑phosphorus (CaP and MgP). In addition, high-throughput sequencing analysis demonstrated that the relative abundance of DPAOs involved in P storage increased, wherein the abundance of Acinetobacter and Saprospiraceae rose from 8.0 % and 4.1 % to 16.1 % and 14.0 %. What's more, the highest P recovery efficiency (98.3 ± 1.1 %) could be obtained at optimal conditions for struvite precipitation (pH = 7.56 and P: N: Mg = 1.87:3.66:1) through the response surface method (RSM) simulation, and the precipitates test analysis indicated that P recovery from biofilm sludge was potentially operable. This research was of great essentiality for exploring the recovery of P from biofilm sludge.

建造了一种新型废水处理厂工艺，以克服同时去除硝酸盐和回收磷 (P) 的挑战。结果表明，运行136天后，磷和硝酸盐去除率从39.0%和48.4%上升到92.8%和93.6%，生物膜中总磷含量（TP_生物膜）从15.8 mg/g SS上升到57.8毫克/克SS。_{此外，TP生物膜}的增加改变了反硝化聚磷酸盐积累生物（DPAOs）的代谢模式，使富集水流的P浓度增加至172.5 mg/L。此外，酸/碱发酵导致细胞膜破裂，释放出DPAO中细胞/EPS的多磷酸盐和正磷酸盐，并释放金属磷（Ca P和Mg P）。此外，高通量测序分析表明，参与储磷的DPAO相对丰度增加，其中不动杆菌和腐螺科的丰度从8.0%和4.1%上升至16.1%和14.0%。更重要的是，通过响应面法（RSM）模拟，在鸟粪石沉淀的最佳条件（pH = 7.56和P：N：Mg = 1.87：3.66：1）下可以获得最高的P回收率（98.3±1.1％），沉淀物测试分析表明，从生物膜污泥中回收磷是可行的。该研究对于探索生物膜污泥中磷的回收具有重要意义。