探索高效环保的信号分子及其群体猝灭能力来控制微囊藻水华,Environmental Science & Technology

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探索高效环保的信号分子及其群体猝灭能力来控制微囊藻水华
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2023-09-04 , DOI: 10.1021/acs.est.3c02395
Ge Yan _{1,

2} , Liang Fu _{1,

2} , Hao Ming _{1,

2} , Congli Chen _{1,

2} , Dandan Zhou _{1,

2}

Affiliation

在全球范围内，蓝藻水华已成为富营养化水体中的严重问题。以前的大多数研究都集中在环境因素上，但忽略了群体感应（QS）在水华发育和控制中的作用。这项研究探索了一种促进细胞生长的关键群体感应分子（QSM），然后提出了一种有针对性的群体猝灭剂来控制水华。通过高分辨率质谱鉴定出一种新的 QSM 3-OH-C4-HSL。人们发现它可以调节细胞碳代谢和能量代谢，作为促进铜绿微囊藻生长的一种手段。为了猝灭3-OH-C4-HSL诱导的QS，根据分子结构提出了三种呋喃酮类抑制剂，其中浓度为20 μM的二氢-3-氨基-2-(3H)-呋喃酮（FN）对铜绿假单胞菌的生长表现出优异的抑制作用（抑制率达 67%）。分子对接分析表明，该抑制剂通过两个氢键（键长分别为3.04和4.04 Å）与Asn164(A)和His167(A)结合，强力占据QSM受体蛋白LuxR，结合能为-5.9 kcal/mol 。该抑制剂阻断信号调节并诱导微囊藻的程序性细胞死亡。重要的是，FN 对水生生物毒性很小，对水生微生物功能的影响可以忽略不计。这项研究为控制蓝藻水华提供了一种有前景的环保新策略。

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Exploring an Efficient and Eco-Friendly Signaling Molecule and Its Quorum Quenching Ability for Controlling Microcystis Blooms

Globally, cyanobacterial blooms have become serious problems in eutrophic water. Most previous studies have focused on environmental factors but have neglected the role of quorum sensing (QS) in bloom development and control. This study explored a key quorum sensing molecule (QSM) that promotes cell growth and then proposed a targeted quorum quencher to control blooms. A new QSM 3-OH-C4-HSL was identified with high-resolution mass spectrometry. It was found to regulate cellular carbon metabolism and energy metabolism as a means to promote Microcystis aeruginosa growth. To quench the QS induced by 3-OH-C4-HSL, three furanone-like inhibitors were proposed based on molecular structure, of which dihydro-3-amino-2-(3H)-furanone (FN) at a concentration of 20 μM exhibited excellent inhibition of M. aeruginosa growth (by 67%). Molecular docking analysis revealed that the inhibitor strongly occupied the QSM receptor protein LuxR by binding with Asn164(A) and His167(A) via two hydrogen bonds (the bond lengths were 3.04 and 4.04 Å) and the binding energy was −5.9 kcal/mol. The inhibitor blocked signaling regulation and induced programmed cell death in Microcystis. Importantly, FN presented little aquatic biotoxicity and negligibly affected aquatic microbial function. This study provides a promising new and eco-friendly strategy for controlling cyanobacterial blooms.

更新日期：2023-09-04

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