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Fluorescence detection of hydrazine in an aqueous environment by a corrole derivative
Luminescence ( IF 3.2 ) Pub Date : 2023-08-31 , DOI: 10.1002/bio.4589 Guifen Lu 1 , Siyuan Yu 1 , Luyao Duan 1 , Suci Meng 1, 2 , Sihan Ding 1 , Ting Dong 1
Luminescence ( IF 3.2 ) Pub Date : 2023-08-31 , DOI: 10.1002/bio.4589 Guifen Lu 1 , Siyuan Yu 1 , Luyao Duan 1 , Suci Meng 1, 2 , Sihan Ding 1 , Ting Dong 1
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Broadly, the industrial applications of hydrazine cause environmental pollution and damage to living organisms because of the high toxicity of hydrazine. Therefore, monitoring hydrazine in the environmental system is of great significance to human health. Here, a new fluorescent probe PC-N2H4 based on corrole dye was developed for the detection of hydrazine that had excellent specificity, low limit of detection (LOD: 88 nM), and a wide pH range (6–12). Upon addition of hydrazine into the probe solution, the strong red fluorescence was ‘turned on’ centred at 653 nm with a 127-fold fluorescence intensity enhancement. The detection mechanism was proved using ESI-MS, 1H NMR, and density functional theoretical calculations. Importantly, the probe was utilized to fabricate a ready-to-use test strip to realize the visual inspection of hydrazine. Furthermore, PC-N2H4 was successfully applied for practical detection of hydrazine in water samples with satisfactory recoveries ranging from 96.2% to 105.0%, and indicating that the designed PC-N2H4 is highly promising for hydrazine detection in an aqueous environment. Considering the diverse toxicological functions of hydrazine, PC-N2H4 was also successfully used to image exogenous hydrazine in HeLa cells and zebrafish.
中文翻译：
咔咯衍生物荧光检测水环境中的肼
从广义上讲，由于肼的高毒性，肼的工业应用会造成环境污染和对生物体的损害。因此，监测环境系统中的肼对人类健康具有重要意义。在此，开发了一种基于咔咯染料的新型荧光探针PC-N 2 H 4用于检测肼，该探针具有优异的特异性、低检测限（LOD：88 nM）和较宽的pH范围（6-12）。在探针溶液中添加肼后，以 653 nm 为中心的强红色荧光“开启”，荧光强度增强了 127 倍。利用ESI-MS、 1 H NMR和密度泛函理论计算证明了检测机制。重要的是，该探针被用来制作即用型测试条，以实现肼的目视检查。此外，PC-N 2 H 4成功应用于水样中肼的实际检测，回收率令人满意，为 96.2% 至 105.0%，这表明所设计的PC-N 2 H 4在水样中肼的检测方面具有良好的应用前景。环境。考虑到肼的多种毒理学功能，PC-N 2 H 4也成功用于HeLa细胞和斑马鱼中外源性肼的成像。
更新日期：2023-08-31
中文翻译：
咔咯衍生物荧光检测水环境中的肼
从广义上讲，由于肼的高毒性，肼的工业应用会造成环境污染和对生物体的损害。因此，监测环境系统中的肼对人类健康具有重要意义。在此，开发了一种基于咔咯染料的新型荧光探针PC-N 2 H 4用于检测肼，该探针具有优异的特异性、低检测限（LOD：88 nM）和较宽的pH范围（6-12）。在探针溶液中添加肼后，以 653 nm 为中心的强红色荧光“开启”，荧光强度增强了 127 倍。利用ESI-MS、 1 H NMR和密度泛函理论计算证明了检测机制。重要的是，该探针被用来制作即用型测试条，以实现肼的目视检查。此外，PC-N 2 H 4成功应用于水样中肼的实际检测，回收率令人满意，为 96.2% 至 105.0%，这表明所设计的PC-N 2 H 4在水样中肼的检测方面具有良好的应用前景。环境。考虑到肼的多种毒理学功能，PC-N 2 H 4也成功用于HeLa细胞和斑马鱼中外源性肼的成像。
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