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Gene expression in the liver of the hagfish (Eptatretus burgeri) belonging to the Cyclostomata is ancestral to that of mammals
The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology Pub Date : 2023-08-29 , DOI: 10.1002/ar.25313 Noriaki Ota 1 , Hideaki Kato 2 , Nobuyoshi Shiojiri 3
The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology Pub Date : 2023-08-29 , DOI: 10.1002/ar.25313 Noriaki Ota 1 , Hideaki Kato 2 , Nobuyoshi Shiojiri 3
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Although the liver of the hagfish, an earliest diverged lineage among vertebrates, has a histological architecture similar to that of mammals, its gene expression has not been explored yet. The present study was undertaken to comparatively characterize gene expression in the liver of the hagfish with that of the mouse, using in situ hybridization technique. Expression of alb (albumin) was detectable in all hepatocytes of the hagfish liver, but was negative in intrahepatic bile ducts. Their expression in abundant periportal ductules was weak. The expression pattern basically resembled that in mammalian livers, indicating that the differential expression of hepatocyte markers in hepatocytes and biliary cells may have been acquired in ancestral vertebrates. alb expression was almost homogeneous in the hagfish liver, whereas that in the mouse liver lobule was zonal. The glul (glutamate–ammonia ligase) expression was also homogeneously detectable in hepatocytes without zonation, and weakly so in biliary cells of the hagfish, which contrasted with its restricted pericentral expression in mouse livers. These findings indicated that the hagfish liver did not have mammalian-type zonation. Whereas tetrapods had Hnf (hepatocyte nuclear factor) 1a and Hnf1b genes encoding the transcription factors, the hagfish had a single gene of their orthologue hnf1. Although HNF1α and HNF1β were immunohistochemically detected in hepatocytes and biliary cells of the mouse, respectively, hnf1 was expressed in both hepatocytes and biliary cells of the hagfish. These data indicate that gene expression of hnf1 in the hagfish liver may be ancestral with that of alb and glul during vertebrate evolution.
中文翻译：
环口目盲鳗 (Eptatretus Burgeri) 肝脏中的基因表达是哺乳动物的祖先
尽管盲鳗的肝脏是脊椎动物中最早分化的谱系，其组织学结构与哺乳动物相似，但其基因表达尚未得到探索。本研究旨在利用原位杂交技术比较盲鳗肝脏和小鼠肝脏中的基因表达特征。在盲鳗肝脏的所有肝细胞中均可检测到alb （白蛋白）的表达，但在肝内胆管中呈阴性。它们在丰富的门脉周围小管中的表达很弱。该表达模式与哺乳动物肝脏中的表达模式基本相似，表明肝细胞和胆管细胞中肝细胞标志物的差异表达可能是在祖先脊椎动物中获得的。盲鳗肝脏中的alb表达几乎是均匀的，而小鼠肝小叶中的表达是带状的。 glul （谷氨酸-氨连接酶）的表达在无分区的肝细胞中也可均匀检测到，而在盲鳗的胆管细胞中也可检测到较弱的表达，这与小鼠肝脏中其有限的中央周围表达形成鲜明对比。这些发现表明盲鳗肝脏不具有哺乳动物类型的分区。四足动物具有编码转录因子的Hnf （肝细胞核因子） 1a和Hnf1b基因，而盲鳗则具有其直系同源物hnf1的单个基因。虽然免疫组织化学方法分别在小鼠的肝细胞和胆管细胞中检测到 HNF1α 和 HNF1β，但hnf1在盲鳗的肝细胞和胆管细胞中均表达。 这些数据表明，盲鳗肝脏中hnf1的基因表达可能是脊椎动物进化过程中alb和glul的祖先。
更新日期：2023-08-29
中文翻译：
环口目盲鳗 (Eptatretus Burgeri) 肝脏中的基因表达是哺乳动物的祖先
尽管盲鳗的肝脏是脊椎动物中最早分化的谱系，其组织学结构与哺乳动物相似，但其基因表达尚未得到探索。本研究旨在利用原位杂交技术比较盲鳗肝脏和小鼠肝脏中的基因表达特征。在盲鳗肝脏的所有肝细胞中均可检测到alb （白蛋白）的表达，但在肝内胆管中呈阴性。它们在丰富的门脉周围小管中的表达很弱。该表达模式与哺乳动物肝脏中的表达模式基本相似，表明肝细胞和胆管细胞中肝细胞标志物的差异表达可能是在祖先脊椎动物中获得的。盲鳗肝脏中的alb表达几乎是均匀的，而小鼠肝小叶中的表达是带状的。 glul （谷氨酸-氨连接酶）的表达在无分区的肝细胞中也可均匀检测到，而在盲鳗的胆管细胞中也可检测到较弱的表达，这与小鼠肝脏中其有限的中央周围表达形成鲜明对比。这些发现表明盲鳗肝脏不具有哺乳动物类型的分区。四足动物具有编码转录因子的Hnf （肝细胞核因子） 1a和Hnf1b基因，而盲鳗则具有其直系同源物hnf1的单个基因。虽然免疫组织化学方法分别在小鼠的肝细胞和胆管细胞中检测到 HNF1α 和 HNF1β，但hnf1在盲鳗的肝细胞和胆管细胞中均表达。 这些数据表明，盲鳗肝脏中hnf1的基因表达可能是脊椎动物进化过程中alb和glul的祖先。
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