具有受控释放触发静电吸引消除机制的电化学发光传感器，用于群勃龙痕量检测,Analytical Chemistry

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具有受控释放触发静电吸引消除机制的电化学发光传感器，用于群勃龙痕量检测
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-08-30 , DOI: 10.1021/acs.analchem.3c01359
Lu Zhao ₁ , Xiang Ren ₁ , Hongmin Ma ₁ , Huan Wang ₁ , Yuyang Li ₁ , Qin Wei ₁ , Dan Wu ₁ , Huangxian Ju ₁

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控释策略可以通过自开/关模式满足污染物敏感环境监测的需求。在这项工作中，构建了一种具有控释触发静电吸引消除和生物分子刺激响应策略的电化学发光（ECL）生物传感器，以灵敏地检测环境类固醇激素。群勃龙（TB）抗原和抗体之间的特异性结合打开了胺化介孔二氧化硅纳米容器上堵塞的孔。释放的l-半胱氨酸通过-SH和MnO ₂之间的氧化还原反应抵消了MnO ₂ NF表面的负电荷，使得MnO ₂ NF和Ru(dcbpy) ₃²⁺之间的静电相互作用消失。Ru(dcbpy) ₃²⁺在电极上释放ECL信号，从而完成控释触发静电吸引消除策略。此外，以结核抗体为靶标，利用结核抗原与标准物质的竞争策略，利用构建的控释ECL生物传感器对结核标准物质进行检测。此外，MnO ₂ NFs作为ECL生物传感器的底物，增加了电极的活性比表面积，有效催化OH ^·和O ₂^·–的产生，从而赋予了ECL生物传感器共反应催化增强特性，进一步提高了其性能。 ECL 性能。这种灵敏的信号响应使所构建的ECL生物传感器的检测限低至2.53 fg/mL，为环境中污染物的高效痕量分析提供了可行的思路。

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Electrochemiluminescence Sensor with Controlled-Release Triggering Electrostatic Attraction Elimination Mechanism for Trenbolone Trace Detection

A controlled-release strategy can meet the needs of sensitive environmental monitoring for pollutants through a self-on/off mode. In this work, an electrochemiluminescence (ECL) biosensor with controlled-release triggering electrostatic attraction elimination and biomolecular stimulated response strategies was constructed to detect environmental steroid hormones sensitively. The blocked pores on the aminated mesoporous silica nanocontainers were opened by specific binding between the trenbolone (TB) antigen and the antibody. The released l-cysteine counteracted the negative charge on the MnO₂ NF surface through the redox reaction between –SH and MnO₂, making the electrostatic interaction between the MnO₂ NFs and the Ru(dcbpy)₃²⁺ disappear. Ru(dcbpy)₃²⁺ released an ECL signal on the electrode, thus completing the controlled-release triggering electrostatic attraction elimination strategy. In addition, with the TB antibody as the target and the competition strategy between the TB antigen and the standard substance, the constructed controlled-release ECL biosensor was used to detect the TB standard substance. Moreover, MnO₂ NFs as the substrate of the ECL biosensor increased the active specific surface area of the electrode, effectively catalyzing the production of OH^• and O₂^•–, thus endowing the ECL biosensor with coreactant-catalytic enhancement characteristic and further improving its ECL performance. This sensitive signal response brought about a low limit of detection of 2.53 fg/mL for the constructed ECL biosensor, which contributed a feasible idea for efficient trace analysis of pollutants in the environment.

更新日期：2023-08-30

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