Preclinical in vivo assessment of a cell-free multi-layered scaffold prepared by 3D printing and electrospinning for small-diameter blood vessel tissue engineering in a canine model,Biomaterials Science

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Preclinical in vivo assessment of a cell-free multi-layered scaffold prepared by 3D printing and electrospinning for small-diameter blood vessel tissue engineering in a canine model
Biomaterials Science ( IF 5.8 ) Pub Date : 2023-08-29 , DOI: 10.1039/d3bm00642e
Mehdi Atari ₁ , Abbas Saroukhani ₂ , Maziar Manshaei ₃ , Peiman Bateni ₃ , Anousheh Zargar Kharazi _{1,

4} , Elham Vatankhah ₅ , Shaghayegh Haghjooy Javanmard ₁

Affiliation

Tissue-engineered vascular grafts (TEVGs) are promising alternatives to existing prosthetic grafts. The objective of this study is to evaluate the clinical feasibility of a novel multi-layered small-diameter vascular graft that has a hierarchical structure. Vascular grafts with elaborately designed composition and architecture were prepared by 3D printing and electrospinning and were implanted into the femoral artery of 5 dogs. The patency of the grafts was assessed using Doppler ultrasonography. After 6 months, the grafts were retrieved and histological and SEM examinations were conducted. During implantation, the grafts exhibited resistance to kinking and no blood seepage thanks to the helical structure of the innermost and outermost layers. The grafts showed a high patency rate and remodelling ability. At 6 months post-implantation, the lumen was endothelialized and middle layers were regenerated by infiltration of smooth muscle cells (SMCs) and deposition of extracellular matrix (ECM). These results suggest that the multi-layered vascular graft may be a promising candidate for small-diameter blood vessel tissue engineering in clinical practice.

中文翻译：

通过3D打印和静电纺丝制备的用于犬模型小直径血管组织工程的无细胞多层支架的临床前体内评估

组织工程血管移植物（TEVG）是现有假体移植物的有希望的替代品。本研究的目的是评估具有分层结构的新型多层小直径血管移植物的临床可行性。通过3D打印和静电纺丝制备了具有精心设计的成分和结构的血管移植物，并将其植入5只狗的股动脉中。使用多普勒超声检查评估移植物的通畅性。6个月后，取出移植物并进行组织学和扫描电镜检查。在植入过程中，由于最内层和最外层的螺旋结构，移植物表现出抗扭结且无血液渗漏。移植物表现出较高的通畅率和重塑能力。植入后 6 个月，管腔内皮化，中间层通过平滑肌细胞 (SMC) 的浸润和细胞外基质 (ECM) 的沉积而再生。这些结果表明，多层血管移植物可能是临床实践中小直径血管组织工程的有希望的候选者。

更新日期：2023-08-30

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