Reversible detection of bisulfite (HSO₃^-) and hydrogen peroxide (H₂O₂) is highly desirable in biology and food safety owing to their redox pair function in biological systems and their utilization in food industry. In this work, a ratiometric fluorescent probe (TPA-Y) with aggregation-induced emission (AIE) properties based on triphenylamine fluorophore was designed and synthesized for reversibly monitoring of HSO₃^-/H₂O₂. Reaction of HSO₃^- with TPA-Y can result in ratiometric fluorescence changes (I₄₄₄/I₆₂₉), and the emission spectrum blue shift reaches 185 nm, which can effectively avoid spectral overlap. Meanwhile, the solution color changed obviously from orange to colorless, enabling naked eye detection of HSO₃^-. TPA-Y was capable of quantitatively detect HSO₃^- in sugar and wine samples with the recovery rates ranging from 94.52 to 102.18%. In addition, TPA-Y is suitable to prepare paper strips and anti-counterfeiting ink for HSO₃^- detection. In addition, the original TPA-Y can be released after the addition product (TPA-Y-HSO₃^-) is oxidized by H₂O₂, and the HSO₃^-/H₂O₂ redox cycle can repeat for at least six times. The probe TPA-Y can be applied to fluorescence imaging of reversible and dynamic interconversion between HSO₃^- and H₂O₂ in living MCF-7 cells.

由于亚硫酸氢盐 (HSO ₃ ^- ) 和过氧化氢 (H ₂ O ₂ ) 在生物系统中的氧化还原对功能及其在食品工业中的应用，亚硫酸氢盐 (HSO 3 - ) 和过氧化氢 (H 2 O 2 ) 的可逆检测在生物学和食品安全中非常理想。本工作设计并合成了一种基于三苯胺荧光团的具有聚集诱导发射(AIE)特性的比例荧光探针( TPA-Y )，用于可逆监测HSO ₃ ^- /H ₂ O ₂。HSO ₃ ^-与TPA-Y的反应可导致比率荧光变化 ( I ₄₄₄ / I₆₂₉），发射光谱蓝移达到185 nm，可以有效避免光谱重叠。同时，溶液颜色明显从橙色变为无色，可以肉眼检测到HSO ₃ ^-。TPA-Y能够定量检测糖和酒样品中的HSO ₃ ^-，回收率在94.52%~102.18%之间。此外，TPA-Y适合制备用于HSO ₃ ^-检测的纸条和防伪油墨。另外，加成产物( TPA-Y-HSO ₃ ^- )被H ₂ O氧化后，可以释放出原来的TPA-Y。_如图2所示，HSO ₃ ^- /H ₂ O ₂氧化还原循环可以重复至少六次。探针TPA-Y可应用于活体MCF-7细胞中HSO ₃ ^-和H ₂ O _{2之间可逆和动态相互转化的荧光成像。}