<br>综合微生物组和多组学分析揭示了 Eisenia fetida 响应生物炭衍生的溶解和颗粒物的分子机制,Journal of Hazardous Materials

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综合微生物组和多组学分析揭示了 Eisenia fetida 响应生物炭衍生的溶解和颗粒物的分子机制
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2023-08-28 , DOI: 10.1016/j.jhazmat.2023.132422
Huiting Jia ₁ , Yuanyuan Zhao ₁ , Hui Deng ₁ , Huamei Yu ₁ , Chengjun Ge ₁ , Jiatong Li ₁

Affiliation

目前，大多数生态毒理学研究仍然局限于关注生物炭本身对土壤动物群的有害影响。然而，从生物炭分离到陆生无脊椎动物的不同成分的潜在生态毒性仍然知之甚少。本研究将溶解物（DM）和颗粒物（PM）与生物炭（BC）分离，然后引入土壤-蚯蚓系统，从分子水平上研究蚯蚓的响应机制。结果表明，BC 和 DM 暴露导致铸型细菌群落中变形菌门丰度增加，提示肠道菌群失调。还观察到，铸型细菌群落对 DM 暴露比 PM 暴露更敏感。转录组学分析显示，BC 和 DM 暴露诱导了与感染和神经性疾病相关的功能通路的显着富集。代谢组学分析显示，DM 暴露导致代谢功能障碍、抗氧化和解毒能力下降。此外，蚯蚓在转录组和代谢水平的反应的显着差异证实 DM 表现出比 PM 更大的生态毒性。本研究从转录组学和代谢组学特征的角度强调了溶解物质对生物炭生态毒性的重大贡献。

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Integrated microbiome and multi-omics analysis reveal the molecular mechanisms of Eisenia fetida in response to biochar-derived dissolved and particulate matters

At present, most ecotoxicological studies are still confined to focusing on the harmful effects of biochar itself on soil fauna. However, the potential ecotoxicity of different components separated from biochar to terrestrial invertebrates remains poorly understood. In this study, the dissolved matter (DM) and particulate matter (PM) were separated from biochar (BC) and then introduced into the soil-earthworm system to investigate the response mechanism of earthworms at the molecular level. The results showed that BC and DM exposure caused an increase in the abundance of Proteobacteria in the cast bacterial community, suggesting the dysbiosis of intestinal microbiota. It was also observed that the cast bacterial communities were more sensitive to DM exposure than PM exposure. Transcriptomic analysis showed that BC and DM exposure induced significant enrichment of functional pathways related to infectious and neuropathic diseases. Metabolomic profiling manifested that DM exposure caused metabolic dysfunction, antioxidant and detoxification abilities recession. Furthermore, significant differences in the responses of earthworms at transcriptomic and metabolic levels confirmed that DM exhibited greater ecotoxicity than PM. This study highlighted the significant contributions of dissolved matter to the ecotoxicity of biochar from the perspective of transcriptomic and metabolomic profiles.

更新日期：2023-08-28

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