Natural Products and Bioprospecting ( IF 4.8 ) Pub Date : 2023-08-28 , DOI: 10.1007/s13659-023-00393-0 Shu-Hui Wang 1 , Cong Guo 1 , Wen-Jin Cui 1 , Qing-Xia Xu 1 , Jun Zhang 1 , Jin-Zhu Jiang 1 , Yan Liu 1 , Sha Chen 1 , Chang Chen 1 , Jin-Tang Cheng 1 , An Liu 1
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An undescribed pyrrole acid, 1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid (1) and one known pyrrole acid (2) were isolated from the fruits of Phyllanthus emblica. The structures of these compounds were elucidated via the comprehensive analyses of IR, HRESIMS, 1D and 2D spectroscopic data. A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide (NO), interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1) and tumor necrosis factor-α (TNF-α) by reducing the phosphorylation of extracellular regulated protein kinases (ERK) and c-Jun N-terminal kinases (JNK) in RAW 264.7 cells. Additionally, compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of ATP-binding cassette transporter A1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.
Graphical abstract
中文翻译：
余甘子中分离的两种吡咯酸及其生物活性
从余甘子的果实中分离出未描述的吡咯酸、1-(4'-甲氧基-4'-氧代丁基)-1H- 吡咯-2,5-二甲酸( 1 )和一种已知的吡咯酸( 2 )。通过IR、HRESIMS、一维和二维光谱数据的综合分析，阐明了这些化合物的结构。一系列生物学测定表明，化合物1和2可以抑制 LPS 诱导的一氧化氮 (NO)、白细胞介素 6 (IL-6)、单核细胞趋化蛋白 1 (MCP-1) 和肿瘤坏死因子 -α 的过度产生。 (TNF- α ) 通过减少 RAW 264.7 细胞中细胞外调节蛋白激酶 (ERK) 和 c-Jun N 末端激酶 (JNK) 的磷酸化。此外，发现化合物1和2可减少氧化低密度脂蛋白处理的 RAW264.7 巨噬细胞中的脂质沉积并增加 ATP 结合盒转运蛋白 A1 的 mRNA 表达。
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