In this study, new imidazole-2,3-dihydrothiazole derivatives were synthesized. It has been noticed that there are few studies on the direction in which the thiazole ring is closed using a thiourea residue. For this purpose, two-dimensional NMR analyses (HMBC, HSQC, and NOESY) were performed for the obtained compounds. The antifungal activities of the compounds were evaluated in vitro using an EUCAST protocol. Compound 2d showed activity against the C. parapsilosis strain with MIC₅₀ = 0.98 μg mL⁻¹, making it the most potent derivative in the series. In addition, compound 2d inhibited ergosterol biosynthesis by 87.953%. Molecular docking and molecular dynamics simulations performed using compounds 2d and 2e are in harmony with activity studies.

中文翻译：

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作为麦角甾醇生物合成抑制剂的新型噻唑-2(3H)-亚胺衍生物的合成，并使用二维核磁共振技术阐明其结构

本研究合成了新的咪唑-2,3-二氢噻唑衍生物。已经注意到，关于使用硫脲残基封闭噻唑环的方向的研究很少。为此，对所得化合物进行了二维 NMR 分析（HMBC、HSQC 和 NOESY）。使用 EUCAST 方案在体外评估化合物的抗真菌活性。化合物2d显示出针对C. parapsilosis菌株的活性，MIC ₅₀ = 0.98 μg mL ^-1，使其成为该系列中最有效的衍生物。此外，化合物2d抑制麦角甾醇生物合成87.953%。使用化合物2d和2e进行的分子对接和分子动力学模拟与活性研究一致。