Alternative splicing of chitin deacetylase 2 regulates chitin and fatty acid metabolism in Asian citrus psyllid, Diaphorina citri,Archives of Insect Biochemistry and Physiology

当前位置： X-MOL 学术 › Arch. Insect Biochem. Physiol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Alternative splicing of chitin deacetylase 2 regulates chitin and fatty acid metabolism in Asian citrus psyllid, Diaphorina citri
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2023-08-25 , DOI: 10.1002/arch.22050
Qin Zhang ₁ , Tao Xia ₁ , Ai-Yun Wang ₂ , Yan Liu ₁ , Ning-Yan Li ₁ , Long Yi _{1,

3} , Zhan-Jun Lu _{1,

3} , Hai-Zhong Yu _{1,

3}

Affiliation

Chitin plays an important role in the development and molting of insects. The key genes involved in chitin metabolism were considered promising targets for pest control. In this study, two splice variants of chitin deacetylase 2 (CDA2) from Diaphorina citri were identified, including DcCDA2a and DcCDA2b. Bioinformatics analysis revealed that DcCDA2a and DcCDA2b encoded 550 and 544 amino acid residues with a signal peptide, respectively. Spatio-temporal expression patterns analysis showed that DcCDA2a and DcCDA2b were highly expressed in D. citri wing and nymph stages. Moreover, DcCDA2a and DcCDA2b expression levels were induced by 20-hydroxyecdysone (20E). Silencing DcCDA2a by RNA interference (RNAi) significantly disrupted the D. citri molting and increased D. citri mortality and malformation rate, whereas inhibition of DcCDA2b resulted in a semimolting phenotype. Furthermore, silencing DcCDA2a and DcCDA2b significantly suppressed D. citri chitin and fatty acid metabolism. Our results indicated that DcCDA2 might play crucial roles in regulating D. citri chitin and fatty acid metabolism, and it could be used as a potential target for controlling D. citri.

中文翻译：

几丁质脱乙酰酶 2 的选择性剪接调节亚洲柑橘木虱 (Diaphorina citri) 的几丁质和脂肪酸代谢

甲壳素在昆虫的发育和蜕皮中起着重要作用。参与几丁质代谢的关键基因被认为是害虫控制的有希望的目标。在这项研究中，鉴定了柑橘木甲甲壳素脱乙酰酶 2 ( CDA2 )的两个剪接变体，包括DcCDA2a和DcCDA2b。生物信息学分析表明，DcCDA2a和DcCDA2b分别编码550和544个氨基酸残基和信号肽。时空表达模式分析表明，DcCDA2a和DcCDA2b在柑橘果蝇翅期和若虫期高表达。此外，20-羟基蜕皮激素（20E）诱导DcCDA2a和DcCDA2b表达水平。通过RNA干扰（RNAi）沉默DcCDA2a会显着破坏柑橘皮蝇的蜕皮并增加柑橘皮蝇的死亡率和畸形率，而抑制DcCDA2b会导致半蜕皮表型。此外，沉默DcCDA2a和DcCDA2b显着抑制D. citri几丁质和脂肪酸代谢。我们的结果表明，DcCDA2可能在调节柑橘夜蛾几丁质和脂肪酸代谢中发挥重要作用，可作为控制柑橘夜蛾的潜在靶点。

更新日期：2023-08-25

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南