Down-regulation of iron/zinc ion transport and toxin synthesis in Microcystis aeruginosa exposed to 5,4′-dihydroxyflavone,Journal of Hazardous Materials

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Down-regulation of iron/zinc ion transport and toxin synthesis in Microcystis aeruginosa exposed to 5,4′-dihydroxyflavone
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2023-08-25 , DOI: 10.1016/j.jhazmat.2023.132396
Shumiao Yu ₁ , Caicai Xu ₂ , Tao Tang ₂ , Yiyi Zhang ₂ , Kokoette Effiong ₂ , Jing Hu ₂ , Yonghong Bi ₃ , Xi Xiao ₄

Affiliation

Flavonoids, common natural polyphenolic compounds from plants, have been proposed as highly effective and safe algicides. However, the molecular mechanism of flavonoids inhibiting Microcystis aeruginosa remains unclear. This study aims in exploring the global transcriptional changes and molecular docking in cyanobacterial cells in response to flavonoids. Transcriptomic analysis revealed that 5,4′-dihydroxyflavone (DHF) primarily affected the genes transcription of iron and zinc ion transport, resulting in the blockage of transport for iron (II), iron (III) and zinc (II), which eventually led to a decrease in intracellular iron and zinc content. 5,4′-DHF can also interfere with iron and zinc transport by binding to metal ion transport-related proteins, leading to eliminated biological activities in M. aeruginosa. Meanwhile, 5,4′-DHF inhibit microcystin synthesis and reduce the content of intercellular toxin by inhibiting the transcription of mcyC and binding with McyC protein, implying that 5,4′-DHF have potential to reduce the risk of microcystins in the environment. Moreover, iron starvation and down-regulation of photosynthesis-related genes transcription led to the inhibition of electron transport in photosynthetic system. These results provide more information for the inhibitory mechanism of flavonoids, and the inhibition of flavonoids on metal ion transmembrane transport provides a new perspective for the development of allelochemical algicides.

中文翻译：

下调暴露于 5,4′-二羟基黄酮的铜绿微囊藻中铁/锌离子转运和毒素合成

类黄酮是来自植物的常见天然多酚化合物，已被提议作为高效和安全的灭藻剂。然而，黄酮类化合物抑制铜绿微囊藻的分子机制仍不清楚。本研究旨在探讨蓝藻细胞响应类黄酮的整体转录变化和分子对接。转录组学分析显示，5,4′-二羟基黄酮（DHF）主要影响铁和锌离子转运的基因转录，导致铁（II）、铁（III）和锌（II）的转运受阻，最终导致细胞内铁和锌含量降低。5,4′-DHF 还可以通过与金属离子转运相关蛋白结合来干扰铁和锌的转运，从而导致铜绿分枝杆菌的生物活性被消除。同时，5,4′-DHF 通过抑制 mcyC 的转录并与 McyC 蛋白结合来抑制微囊藻毒素合成并降低细胞间毒素的含量，这意味着 5,4′-DHF 有可能降低环境中微囊藻毒素的风险。此外，铁饥饿和光合作用相关基因转录的下调导致光合作用系统中电子传递的抑制。这些结果为黄酮类化合物的抑制机制提供了更多信息，黄酮类化合物对金属离子跨膜转运的抑制为化感化学灭藻剂的开发提供了新的视角。

更新日期：2023-08-25

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