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Exceptional peroxidase-like activity of an iron and copper based organic framework nanosheet for consecutive colorimetric biosensing of glucose and kanamycin in real food samples
Analyst ( IF 3.6 ) Pub Date : 2023-08-25 , DOI: 10.1039/d3an01242e Rajakumari Jesuraj 1 , Arunjegan Amalraj 1 , Vinoth Kumar Vaidyanathan 2 , Panneerselvam Perumal 1
Analyst ( IF 3.6 ) Pub Date : 2023-08-25 , DOI: 10.1039/d3an01242e Rajakumari Jesuraj 1 , Arunjegan Amalraj 1 , Vinoth Kumar Vaidyanathan 2 , Panneerselvam Perumal 1
Affiliation
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Two-dimensional metal–organic framework nanosheets are attractive as peroxidase mimicking nanocatalysts due to their rich chemical functional groups, large surface area, high porosity, and accessible active sites. In this study, we synthesized FeCu bifunctional 2D MOF nanosheets using a solvothermal method. Fe and Cu ions were added as metal precursors, while organic amine and acid served as the organic ligands to construct the FeCu-MOF nanosheets. These nanosheets demonstrated robust peroxidase-like catalytic activities and were employed to develop a visual detection system for multiple targets, such as glucose and kanamycin. In the detection mechanism, glucose was oxidized into gluconic acid by glucose oxidase (GOx), leading to the generation of H2O2. When H2O2 is present, the FeCu-MOF NSs demonstrate high intrinsic peroxidase-like activity, which might catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) into a blue-coloured oxTMB product with a strong UV absorption at 654 nm. Subsequently, kanamycin was added to the above sensing system. The kanamycin strongly interacted with the FeCu-MOF NSs through H-bonding and blocked electron transfer, resulting in a colour change of the solution from blue to colourless with a weak UV absorption at 654 nm. Under the optimal conditions, the proposed colorimetric sensor exhibits an excellent linear response to glucose and kanamycin over the 0.25–5 μM and 0.02–0.1 μM ranges, respectively. The proposed colorimetric assay detection limits for glucose and kanamycin were found to be as low as 0.1 μM and 8 nM, respectively, and such a sensor shows excellent selectivity and sensitivity against different potential interferents. Thus, our proposed colorimetric assay was satisfactory when applied to glucose and kanamycin detection in agricultural and livestock husbandry samples.
中文翻译：
铁和铜基有机框架纳米片具有出色的类过氧化物酶活性，可对真实食品样品中的葡萄糖和卡那霉素进行连续比色生物传感
二维金属有机骨架纳米片由于其丰富的化学官能团、大表面积、高孔隙率和易接近的活性位点，作为过氧化物酶模拟纳米催化剂而具有吸引力。在这项研究中，我们采用溶剂热法合成了 FeCu 双功能二维 MOF 纳米片。添加 Fe 和 Cu 离子作为金属前体，而有机胺和酸作为有机配体来构建 FeCu-MOF 纳米片。这些纳米片表现出强大的类似过氧化物酶的催化活性，并用于开发针对多个目标（例如葡萄糖和卡那霉素）的视觉检测系统。在检测机制中，葡萄糖被葡萄糖氧化酶(GOx)氧化成葡萄糖酸，从而产生H 2 O 2。当 H 2 O 2存在时，FeCu-MOF NSs 表现出较高的内在过氧化物酶样活性，可能会催化氧化 3,3',5,5'-四甲基联苯胺 (TMB) 生成具有强紫外光的蓝色 oxTMB 产物654 nm 处的吸收。随后，将卡那霉素添加到上述传感系统中。卡那霉素通过氢键与 FeCu-MOF NS 发生强烈相互作用并阻断电子转移，导致溶液颜色从蓝色变为无色，并在 654 nm 处有微弱的紫外吸收。在最佳条件下，所提出的比色传感器分别在 0.25-5 μM 和 0.02-0.1 μM 范围内对葡萄糖和卡那霉素表现出出色的线性响应。所提出的葡萄糖和卡那霉素的比色测定检测限分别低至 0.1 μM 和 8 nM，并且这种传感器对不同的潜在干扰物表现出优异的选择性和灵敏度。因此，我们提出的比色测定法应用于农业和畜牧业样品中的葡萄糖和卡那霉素检测时是令人满意的。
更新日期：2023-08-25
中文翻译：
铁和铜基有机框架纳米片具有出色的类过氧化物酶活性，可对真实食品样品中的葡萄糖和卡那霉素进行连续比色生物传感
二维金属有机骨架纳米片由于其丰富的化学官能团、大表面积、高孔隙率和易接近的活性位点，作为过氧化物酶模拟纳米催化剂而具有吸引力。在这项研究中，我们采用溶剂热法合成了 FeCu 双功能二维 MOF 纳米片。添加 Fe 和 Cu 离子作为金属前体，而有机胺和酸作为有机配体来构建 FeCu-MOF 纳米片。这些纳米片表现出强大的类似过氧化物酶的催化活性，并用于开发针对多个目标（例如葡萄糖和卡那霉素）的视觉检测系统。在检测机制中，葡萄糖被葡萄糖氧化酶(GOx)氧化成葡萄糖酸，从而产生H 2 O 2。当 H 2 O 2存在时，FeCu-MOF NSs 表现出较高的内在过氧化物酶样活性，可能会催化氧化 3,3',5,5'-四甲基联苯胺 (TMB) 生成具有强紫外光的蓝色 oxTMB 产物654 nm 处的吸收。随后，将卡那霉素添加到上述传感系统中。卡那霉素通过氢键与 FeCu-MOF NS 发生强烈相互作用并阻断电子转移，导致溶液颜色从蓝色变为无色，并在 654 nm 处有微弱的紫外吸收。在最佳条件下，所提出的比色传感器分别在 0.25-5 μM 和 0.02-0.1 μM 范围内对葡萄糖和卡那霉素表现出出色的线性响应。所提出的葡萄糖和卡那霉素的比色测定检测限分别低至 0.1 μM 和 8 nM，并且这种传感器对不同的潜在干扰物表现出优异的选择性和灵敏度。因此，我们提出的比色测定法应用于农业和畜牧业样品中的葡萄糖和卡那霉素检测时是令人满意的。
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