Molecular Horticulture Pub Date : 2023-08-25 , DOI: 10.1186/s43897-023-00063-2 Kexin Xu 1 , Dongdong Tian 2 , TingJin Wang 1 , Aijun Zhang 1 , Mohamed Abdou Youssef Elsadek 1 , Weihong Liu 1 , Liping Chen 1 , Yongfeng Guo 2
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Tomato (Solanum lycopersicum) is one of the most important vegetable crops in the world and abiotic stresses often cause serious problems in tomato production. It is thus important to identify new regulators in stress response and to devise new approaches to promote stress tolerance in tomato. Previous studies have shown that small secreted peptides (SSPs) are important signal molecules regulating plant growth and stress response by mediating intercellular communication. However, little is known about tomato SSPs, especially their roles in responding to abiotic stresses. Here we report the identification of 1,050 putative SSPs in the tomato genome, 557 of which were classified into 38 known SSP families based on their conserved domains. GO and transcriptome analyses revealed that a large proportion of SlSSPs might be involved in abiotic stress response. Further analysis indicated that stress response related cis-elements were present on the SlCEP promotors and a number of SlCEPs were significantly upregulated by drought treatments. Among the drought-inducible SlCEPs, SlCEP10 and SlCEP11b were selected for further analysis via exogenous application of synthetic peptides. The results showed that treatments with both SlCEP10 and SlCEP11b peptides enhanced tomato drought stress tolerance, indicating the potential roles of SlSSPs in abiotic stress response.
Graphical Abstract
中文翻译：
番茄中的小分泌肽（SSP）及其在干旱胁迫响应中的潜在作用
番茄（Solanum lycopersicum）是世界上最重要的蔬菜作物之一，非生物胁迫常常给番茄生产带来严重问题。因此，识别应激反应中的新调节因子并设计新方法来促进番茄的应激耐受性非常重要。先前的研究表明，小分泌肽（SSP）是通过介导细胞间通讯来调节植物生长和胁迫反应的重要信号分子。然而，人们对番茄 SSP 知之甚少，尤其是它们在应对非生物胁迫方面的作用。在这里，我们报告了番茄基因组中 1,050 个假定的 SSP 的鉴定，其中 557 个根据其保守结构域被分为 38 个已知的 SSP 家族。 GO 和转录组分析表明，大部分SlSSP可能参与非生物胁迫反应。进一步分析表明， SlCEP启动子上存在与胁迫反应相关的顺式元件，并且许多SlCEP因干旱处理而显着上调。在干旱诱导型SlCEP中，选择 SlCEP10 和 SlCEP11b 通过外源应用合成肽进行进一步分析。结果表明，SlCEP10 和 SlCEP11b 肽处理增强了番茄的干旱胁迫耐受性，表明 SlSSP 在非生物胁迫响应中的潜在作用。
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