Ciprofloxacin (CIP) and norfloxacin (NOR) belong to the class of emerging contaminants that are frequently detected in the aquatic environment as a binary mixture, responsible for the development of antibiotic-resistant genes and antibiotic-resistant bacteria. This study aims to investigate five different algal species Chlorella vulgaris (Cv), Chlorella pyrenoidosa (Cp), Scenedesmus obliquus (So), Tetradesmus sp (T) and Monoraphidium sp (M) for their tolerance and removal of binary mixture. The effects on biochemical composition in the algal species concerning the binary mixture and its removal efficiency are first reported in this study. The acute toxicity (96 h EC₅₀) values are in the order of So > Cp > T > M > Cv, Chlorella vulgaris is the most sensitive algal species with 17.73 ± 0.24 mg/L and Scenedesmus obliquus is the least sensitive algal species with 39.19 ± 0.79 mg/L. The removal efficiency of the binary mixture was found to be in the order of So > Cp > T > M > Cv, Scenedesmus obliquus removed CIP (52.4%) and NOR (87.5%) with biodegradation as the major contributing removal mechanism. Furthermore, less toxic biotransformed products were detected in Scenedesmus obliquus and the biochemical characterization revealed that the growth-stimulating effect is higher with lipid (35%), carbohydrate (18%), and protein (33%) providing an advantage in the production of valuable biomass.

环丙沙星 (CIP) 和诺氟沙星 (NOR) 属于一类新兴污染物，经常以二元混合物的形式在水生环境中检测到，导致抗生素抗性基因和抗生素抗性细菌的产生。本研究旨在调查五种不同的藻类：普通小球藻( Cv )、蛋白核小球藻( Cp )、斜生栅藻( So )、四藻( T ) 和单针藻( M ) 对二元混合物的耐受性和去除能力。本研究首次报道了二元混合物对藻类生化成分的影响及其去除效率。急性毒性（96 h EC ₅₀）值的顺序为So  >  Cp  >  T  >  M  >  Cv，小球藻是最敏感的藻类，为17.73 ± 0.24 mg/L，栅藻是最不敏感的藻类，为17.73 ± 0.24 mg/L。 39.19±0.79毫克/升。二元混合物的去除效率按So  >  Cp  >  T  >  M  >  Cv的顺序排列，斜生栅藻去除了 CIP (52.4%) 和 NOR (87.5%)，其中生物降解是主要的去除机制。此外，在斜生栅藻中检测到毒性较低的生物转化产物，生化特征表明，脂质（35%）、碳水化合物（18%）和蛋白质（33%）的生长刺激作用较高，在生产中具有优势。有价值的生物质。