Corals are under siege by both local and global threats, creating a worldwide reef crisis. Cryopreservation is an important intervention measure and a vital component of the modern coral conservation toolkit, but preservation techniques are currently limited to sensitive reproductive materials that can only be obtained a few nights per year during spawning. Here, we report the successful cryopreservation and revival of cm-scale coral fragments via mL-scale isochoric vitrification. We demonstrate coral viability at 24 h post-thaw using a calibrated oxygen-uptake respirometry technique, and further show that the method can be applied in a passive, electronics-free configuration. Finally, we detail a complete prototype coral cryopreservation pipeline, which provides a platform for essential next steps in modulating post-thaw stress and initiating long-term growth. These findings pave the way towards an approach that can be rapidly deployed around the world to secure the biological genetic diversity of our vanishing coral reefs.

珊瑚受到当地和全球威胁的围困，造成了全球珊瑚礁危机。低温保存是一项重要的干预措施，也是现代珊瑚保护工具包的重要组成部分，但保存技术目前仅限于敏感的繁殖材料，这些材料每年只能在产卵期间的几个晚上获得。在这里，我们报告了通过毫升级等容玻璃化成功冷冻保存和复活厘米级珊瑚碎片。我们使用校准的摄​​氧量呼吸测量技术展示了解冻后 24 小时的珊瑚活力，并进一步表明该方法可以应用于被动、无电子配置。最后，我们详细介绍了一个完整的珊瑚低温保存管道原型，它为调节解冻后压力和启动长期生长的重要后续步骤提供了一个平台。这些发现为一种可以在世界各地快速部署的方法铺平了道路，以确保正在消失的珊瑚礁的生物遗传多样性。