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Development of Novel Peptide-Modified Silver Nanoparticle-Based Rapid Biosensors for Detecting Aminoglycoside Antibiotics
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-08-21 , DOI: 10.1021/acs.jafc.3c03565 Dae-Young Kim 1 , Sanjeev K Sharma 2 , Kashif Rasool 3 , Janardhan Reddy Koduru 4 , Asad Syed 5 , Gajanan Ghodake 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-08-21 , DOI: 10.1021/acs.jafc.3c03565 Dae-Young Kim 1 , Sanjeev K Sharma 2 , Kashif Rasool 3 , Janardhan Reddy Koduru 4 , Asad Syed 5 , Gajanan Ghodake 1
Affiliation
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The detection and monitoring of aminoglycoside antibiotics (AGAs) have become of utmost importance due to their widespread use in human and animal therapy, as well as the associated risks of exposure, toxicity, and the emergence of antimicrobial resistance. In this study, we successfully synthesized casein hydrolysate peptides-functionalized silver nanoparticles (CHPs@AgNPs) and employed them as a novel colorimetric analytical platform to demonstrate remarkable specificity and sensitivity toward AGAs. The colorimetric and spectral response of the CHPs@AgNPs was observed at 405 and 520 nm, showing a linear correlation with the concentration of streptomycin, a representative AGA. The color changes from yellow to orange provided a visual indication of the analyte concentration, enabling quantitative determination for real-world samples. The AgNP assay exhibited excellent sensitivity with dynamic ranges of approximately 200–650 and 100–700 nM for streptomycin-spiked tap water and dairy whey with limits of detection found to be ∼98 and 56 nM, respectively. The mechanism behind the selective aggregation of CHPs@AgNPs in the presence of AGAs involves the amine groups of the target analytes acting as molecular bridges for electrostatic coupling with hydroxyl or carboxyl functionalities of adjacent NPs, driving the formation of stable NP aggregates. The developed assay offers several advantages, making it suitable for various practical applications. It is characterized by its simplicity, rapidity, specificity, sensitivity, and cost-effectiveness. These unique features make the method a promising tool for monitoring water quality, ensuring food safety, and dealing with emergent issues of antibiotic resistance.
中文翻译：
开发用于检测氨基糖苷类抗生素的新型肽修饰银纳米颗粒快速生物传感器
由于氨基糖苷类抗生素 (AGAs) 在人类和动物治疗中的广泛使用，以及相关的暴露、毒性和抗菌素耐药性风险，其检测和监测变得至关重要。在这项研究中，我们成功合成了酪蛋白水解肽功能化银纳米粒子（CHPs@AgNPs），并将其用作新型比色分析平台，以证明对 AGA 具有显着的特异性和敏感性。在 405 和 520 nm 处观察到 CHP@AgNPs 的比色和光谱响应，显示与代表性 AGA 链霉素的浓度呈线性相关。颜色从黄色变为橙色，提供了分析物浓度的视觉指示，从而能够对真实样品进行定量测定。AgNP 测定对添加链霉素的自来水和乳清表现出出色的灵敏度，动态范围约为 200-650 和 100-700 nM，检测限分别为 ∼98 和 56 nM。在 AGA 存在下，CHP@AgNPs 选择性聚集背后的机制涉及目标分析物的胺基作为分子桥，与相邻 NP 的羟基或羧基官能团进行静电耦合，从而驱动稳定 NP 聚集体的形成。所开发的检测方法具有多种优点，使其适合各种实际应用。其特点是简单、快速、特异性、敏感性和成本效益。这些独特的功能使该方法成为监测水质、确保食品安全和处理紧急抗生素耐药性问题的有前景的工具。
更新日期：2023-08-21
中文翻译：
开发用于检测氨基糖苷类抗生素的新型肽修饰银纳米颗粒快速生物传感器
由于氨基糖苷类抗生素 (AGAs) 在人类和动物治疗中的广泛使用，以及相关的暴露、毒性和抗菌素耐药性风险，其检测和监测变得至关重要。在这项研究中，我们成功合成了酪蛋白水解肽功能化银纳米粒子（CHPs@AgNPs），并将其用作新型比色分析平台，以证明对 AGA 具有显着的特异性和敏感性。在 405 和 520 nm 处观察到 CHP@AgNPs 的比色和光谱响应，显示与代表性 AGA 链霉素的浓度呈线性相关。颜色从黄色变为橙色，提供了分析物浓度的视觉指示，从而能够对真实样品进行定量测定。AgNP 测定对添加链霉素的自来水和乳清表现出出色的灵敏度，动态范围约为 200-650 和 100-700 nM，检测限分别为 ∼98 和 56 nM。在 AGA 存在下，CHP@AgNPs 选择性聚集背后的机制涉及目标分析物的胺基作为分子桥，与相邻 NP 的羟基或羧基官能团进行静电耦合，从而驱动稳定 NP 聚集体的形成。所开发的检测方法具有多种优点，使其适合各种实际应用。其特点是简单、快速、特异性、敏感性和成本效益。这些独特的功能使该方法成为监测水质、确保食品安全和处理紧急抗生素耐药性问题的有前景的工具。
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