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VIGS as a strategy to reverse aphid wing induction by Y-satellite RNA of cucumber mosaic virus
FEBS Open Bio ( IF 2.8 ) Pub Date : 2023-08-19 , DOI: 10.1002/2211-5463.13697
Hangil Kim 1 , Chikara Masuta 1
Affiliation  

Y-satellite RNA (Y-sat) of cucumber mosaic virus upregulates the expression of the aphid ABCG4 gene, which promotes aphid wing formation. We used ABCG4 virus-induced gene silencing (VIGS) to prevent the wing-induction mechanism of Y-sat and thus inhibited aphid wing formation. Of the aphids on plants with VIGS of ABCG4, only about 30% had wings, and 60–70% of the winged aphids were small and likely impaired in flying ability. In addition, we showed that double-stranded RNAs (dsRNAs) and small RNAs were transferred from the plant to the aphid to adequately silence aphid genes. Supplying ABCG4 dsRNA by VIGS to aphids is thus a potential strategy to inhibit aphid wing formation.

中文翻译:

VIGS 作为逆转黄瓜花叶病毒 Y 卫星 RNA 诱导蚜虫翅膀的策略

黄瓜花叶病毒的Y卫星RNA(Y-sat)上调蚜虫ABCG4基因的表达,从而促进蚜虫翅膀的形成。我们利用ABCG4病毒诱导基因沉默(VIGS)来阻止Y-sat的翅诱导机制,从而抑制蚜虫翅的形成。VIGS 为ABCG4的植物上的蚜虫中,只有约 30% 有翅膀,60-70% 的有翅蚜虫体型较小,飞行能力可能受损。此外,我们还发现双链RNA(dsRNA)和小RNA从植物转移到蚜虫中,以充分沉默蚜虫基因。因此,通过 VIGS 向蚜虫提供ABCG4 dsRNA 是抑制蚜虫翅膀形成的潜在策略。
更新日期:2023-08-19
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