Modulated Arabinose Uptake and cAMP Signaling Synergistically Improve Glucose and Arabinose Consumption in Recombinant Yeast,Journal of Agricultural and Food Chemistry

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Modulated Arabinose Uptake and cAMP Signaling Synergistically Improve Glucose and Arabinose Consumption in Recombinant Yeast
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-08-17 , DOI: 10.1021/acs.jafc.3c04386
Jinle Liu _{1,

2} , Junjie Yang ₃ , Lihua Yuan ₄ , Chunhua Wu ₄ , Yu Jiang ₅ , Wei Zhuang _{2,

6} , Hanjie Ying _{2,

6} , Sheng Yang _{3,

5}

Affiliation

During the production of ethanol from lignocellulose-derived sugars, recombinant yeasts tend to utilize xylose and arabinose after glucose exhaustion. So far, many glucose-insensitive pentose transporters have been reported to counteract this phenomenon, but few studies have described intracellular factors. In this study, the combination of adaptive evolution, comparative genomics, and genetic complementation revealed that the hexokinase-deficient (Hxk0) arabinose-fermenting Saccharomyces cerevisiae requires the arabinose transporter variant Gal2-N376T and the mutations of guanine nucleotide exchange factor Cdc25 to overcome glucose restriction during arabinose assimilation. The results showed that the Hxk0 recombinant yeasts could lower the metabolic/physiological threshold of cell proliferation by downregulating the intracellular cAMP levels, resulting in smaller cells and increased arabinose assimilation under glucose restriction. In the medium containing 80 g/L glucose and 20 g/L arabinose, the evolved strain restoring the hexokinase activity completed fermentation at 22 h, compared to 24 h for the parental strain. Overall, the experimental results provide new insights into glucose repression of biorefinery yeasts.

中文翻译：

调节阿拉伯糖摄取和 cAMP 信号传导协同改善重组酵母中的葡萄糖和阿拉伯糖消耗

在从木质纤维素衍生的糖生产乙醇的过程中，重组酵母倾向于在葡萄糖耗尽后利用木糖和阿拉伯糖。迄今为止，许多对葡萄糖不敏感的戊糖转运蛋白已被报道可以抵消这种现象，但很少有研究描述细胞内因素。在这项研究中，适应性进化、比较基因组学和遗传互补的结合揭示了己糖激酶缺陷（Hxk0）的阿拉伯糖发酵酿酒酵母需要阿拉伯糖转运蛋白变体Gal2-N376T和鸟嘌呤核苷酸交换因子Cdc25的突变来克服葡萄糖阿拉伯糖同化过程中的限制。结果表明，Hxk0重组酵母可以通过下调细胞内cAMP水平来降低细胞增殖的代谢/生理阈值，从而导致细胞变小并在葡萄糖限制下增加阿拉伯糖同化。在含有80 g/L葡萄糖和20 g/L阿拉伯糖的培养基中，恢复己糖激酶活性的进化菌株在22小时内完成发酵，而亲本菌株需要24小时。总体而言，实验结果为生物精炼酵母的葡萄糖抑制提供了新的见解。

更新日期：2023-08-17

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