We read with interest the recent paper by Vich Vila et al , which discussed alterations in the metabolome of patients with inflammatory bowel disease (IBD).1 Here, the authors described >300 molecules that were differentially abundant in the faeces of 424 patients with IBD compared with 255 healthy controls, analysed with liquid chromatography with tandem mass spectrometry as well as genome-wide association analysis. Of note, decreased faecal L-urobilin and increased levels of the sphingolipid lactosyl-N-palmitoyl-sphingosine were not only key markers associated with IBD, but furthermore including their ratio improved the accuracy of disease prediction above age, sex, body mass index and faecal calprotectin levels. IBD-associated intestinal dysbiosis is characterised by reduced microbial diversity, increases in facultative anaerobes and a decrease in obligately anaerobic producers of short-chain fatty acids, as well as the accumulation of primary unconjugated bile acids and depletion of secondary bile acids.2 The physiological roles of faecal urobilinoids have not been well described, but alterations may be associated with changes in intestinal bacteria capable of their catabolism such as Bacteroides fragilis and Clostridium ramosum ( Thomasclavelia ramosa ).3 Sphingolipids, produced by the Bacteroidetes phylum, regulate inflammation and immunity via multiple pathways including autophagy, endoplasmic reticulum stress, innate immune responses and G-protein-coupled-receptor signalling.4 Faecal …

中文翻译：

---

关键代谢组学改变与溃疡性结肠炎疾病状态和活动相关：验证分析


我们饶有兴趣地阅读了 Vich Vila 等人最近发表的论文，该论文讨论了炎症性肠病 (IBD) 患者代谢组的变化。1 在此，作者描述了 424 名 IBD 患者粪便中存在丰富程度不同的 > 300 种分子与 255 名健康对照进行比较，采用液相色谱法、串联质谱法以及全基因组关联分析进行分析。值得注意的是，粪便 L-尿胆素的减少和鞘脂乳糖基-N-棕榈酰-鞘氨醇水平的增加不仅是与 IBD 相关的关键标志物，而且还包括它们的比率，提高了疾病预测的准确性，高于年龄、性别、体重指数和粪便钙卫蛋白水平。 IBD 相关肠道菌群失调的特点是微生物多样性减少、兼性厌氧菌增加和短链脂肪酸专性厌氧生产者减少，以及初级非结合胆汁酸的积累和次级胆汁酸的消耗。 2粪便尿胆素的作用尚未得到很好的描述，但这种变化可能与能够进行分解代谢的肠道细菌的变化有关，例如脆弱拟杆菌和梭菌 (Thomasclavelia ramosa)。3 鞘脂由拟杆菌门产生，通过以下方式调节炎症和免疫：多种途径，包括自噬、内质网应激、先天免疫反应和 G 蛋白偶联受体信号传导。4 粪便……