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TET-Yeasate: An engineered yeast whole-cell lysate-based approach for high performance tetracycline degradation
Environment International ( IF 10.3 ) Pub Date : 2023-08-17 , DOI: 10.1016/j.envint.2023.108158 Qian He 1 , Zhuoyu Lin 1 , Meilin Qin 1 , Yu Huang 1 , Zhaoxiang Lu 1 , Mei Zheng 1 , Chaoyue Cui 1 , Cang Li 1 , Xiaojing Zhang 1 , Xiaoping Liao 2 , Yahong Liu 2 , Hao Ren 1 , Jian Sun 2
Environment International ( IF 10.3 ) Pub Date : 2023-08-17 , DOI: 10.1016/j.envint.2023.108158 Qian He 1 , Zhuoyu Lin 1 , Meilin Qin 1 , Yu Huang 1 , Zhaoxiang Lu 1 , Mei Zheng 1 , Chaoyue Cui 1 , Cang Li 1 , Xiaojing Zhang 1 , Xiaoping Liao 2 , Yahong Liu 2 , Hao Ren 1 , Jian Sun 2
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The widespread of tetracycline (TC) residues in anthropogenic and natural environments pose an immediate threat to public health. Herein, we established the TET-Yeasate, an approach based on whole-cell lysate of engineered yeast, to mitigate the TC contamination in environment. The TET-Yeasate is defined as the biological matrix of whole cell lysate from engineered yeast that containing TC-degradative components (Tet(X), NADPH, Mg) and protective macromolecules. The TET-Yeasate was able to efficiently eliminate TC residues in tap water (98.8%), lake water (77.6%), livestock sewage (87.3%) and pharmaceutical wastewater (35.3%) without necessity for exogenous addition of expensive cofactors. The TET-Yeasate was further developed into lyophilized form for ease of storage and delivery. The TET-Yeasate in lyophilized form efficiently removed up to 74.6% TC residue within 0.25 h. In addition, the lyophilization confers promising resilience to TET-Yeasate against adverse temperatures and pH by maintaining degradation efficacy of 85.69%−97.83%. The stability test demonstrated that the biomacromolecules in lysate served as natural protectants that exerted extensive protection on TET-Yeasate during the 14-day storage at various conditions. In addition, 5 potential degradation pathways were elaborated based on the intermediate products. Finally, the analysis indicated that TET-Yeasate enjoyed desirable bio- and eco-safety without introduction of hazardous intermediates and spread of resistance genes. To summary, the TET-Yeasate based on whole cell lysate of engineered yeast provides a cost-effective and safe alternative to efficiently remove TC residues in environment, highlighting the great potential of such whole-cell based methods in environmental decontamination.
中文翻译：
TET-Yeasate：一种基于工程酵母全细胞裂解物的方法，用于高性能四环素降解
人为和自然环境中广泛存在的四环素 (TC) 残留对公众健康构成直接威胁。在此，我们建立了 TET-Yeasate，一种基于工程酵母全细胞裂解物的方法，以减轻环境中的 TC 污染。 TET-Yeasate 被定义为工程酵母全细胞裂解物的生物基质，含有 TC 降解成分（Tet(X)、NADPH、Mg）和保护性大分子。 TET-Yeasate能够有效消除自来水（98.8%）、湖水（77.6%）、牲畜污水（87.3%）和制药废水（35.3%）中的TC残留，而无需外源添加昂贵的辅助因子。 TET-Yeasate 进一步开发为冻干形式，以便于储存和运输。冻干形式的 TET-Yeasate 在 0.25 小时内可有效去除高达 74.6% 的 TC 残留物。此外，冻干法通过维持 85.69%−97.83% 的降解效率，赋予 TET-Yeasate 良好的抵抗不利温度和 pH 值的能力。稳定性测试表明，裂解液中的生物大分子作为天然保护剂，在各种条件下14天的储存过程中对TET-Yeasate发挥了广泛的保护作用。此外，还根据中间产物详细阐述了5种潜在的降解途径。最后，分析表明，TET-Yeasate 具有理想的生物和生态安全性，无需引入危险中间体和传播抗性基因。综上所述，基于工程酵母全细胞裂解液的TET-Yeasate为有效去除环境中的TC残留提供了一种经济有效且安全的替代方案，凸显了这种基于全细胞的方法在环境净化中的巨大潜力。
更新日期：2023-08-17
中文翻译：
TET-Yeasate：一种基于工程酵母全细胞裂解物的方法，用于高性能四环素降解
人为和自然环境中广泛存在的四环素 (TC) 残留对公众健康构成直接威胁。在此，我们建立了 TET-Yeasate，一种基于工程酵母全细胞裂解物的方法，以减轻环境中的 TC 污染。 TET-Yeasate 被定义为工程酵母全细胞裂解物的生物基质，含有 TC 降解成分（Tet(X)、NADPH、Mg）和保护性大分子。 TET-Yeasate能够有效消除自来水（98.8%）、湖水（77.6%）、牲畜污水（87.3%）和制药废水（35.3%）中的TC残留，而无需外源添加昂贵的辅助因子。 TET-Yeasate 进一步开发为冻干形式，以便于储存和运输。冻干形式的 TET-Yeasate 在 0.25 小时内可有效去除高达 74.6% 的 TC 残留物。此外，冻干法通过维持 85.69%−97.83% 的降解效率，赋予 TET-Yeasate 良好的抵抗不利温度和 pH 值的能力。稳定性测试表明，裂解液中的生物大分子作为天然保护剂，在各种条件下14天的储存过程中对TET-Yeasate发挥了广泛的保护作用。此外，还根据中间产物详细阐述了5种潜在的降解途径。最后，分析表明，TET-Yeasate 具有理想的生物和生态安全性，无需引入危险中间体和传播抗性基因。综上所述，基于工程酵母全细胞裂解液的TET-Yeasate为有效去除环境中的TC残留提供了一种经济有效且安全的替代方案，凸显了这种基于全细胞的方法在环境净化中的巨大潜力。
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