近红外光驱动的 MOF@Au 纳米电机用于细菌生物膜的增强穿透和声动力治疗,Chemistry of Materials

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近红外光驱动的 MOF@Au 纳米电机用于细菌生物膜的增强穿透和声动力治疗
Chemistry of Materials ( IF 7.2 ) Pub Date : 2023-08-16 , DOI: 10.1021/acs.chemmater.3c01140
Wei Guo _{1,

2} , Yanmin Wang _{1,

2} , Kai Zhang _{1,

2} , Xiaoguang Dai _{1,

2} , Zhuangzhuang Qiao _{1,

2} , Zhiwen Liu _{1,

2} , Bingran Yu _{1,

2} , Nana Zhao _{1,

2} , Fu-Jian Xu _{1,

2}

Affiliation

声动力疗法（SDT）利用超声波（US）激活声敏剂，产生高细胞毒性的活性氧化物（ROS），在根除深层细菌感染方面取得了巨大成功。然而，声敏剂在生物膜中的有限渗透和低扩散功效严重损害了 SDT 的治疗效果。在此，我们设计了一种具有高电子空穴对分离效率的近红外（NIR）光驱动纳米电机（MOF@Au-DNase I），用于对抗金黄色葡萄球菌（S. aureus ））生物膜。具体来说，将金纳米颗粒（Au NPs）沉积到一半球形金属有机框架（MOF）上构建Janus MOF@Au NPs，然后将脱氧核糖核酸酶（DNase I）共价固定到MOF表面@Au。在近红外激光照射下，MOF@Au NPs表现出高效的主动运动，并在15分钟内快速渗透到深层生物膜中。值得注意的是，DNase I 可以通过水解生物膜中的细胞外 DNA (eDNA) 来破坏生物膜的致密性，以促进声敏剂的渗透。此外，Au的不对称空间分布可以显着提高MOF@Au的电子转移效率，最终增强ROS的产生。这种协同效应提高了 Janus MOF@Au NPs 的 SDT 介导治疗的效率。

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Near-Infrared Light-Propelled MOF@Au Nanomotors for Enhanced Penetration and Sonodynamic Therapy of Bacterial Biofilms

Sonodynamic therapy (SDT) utilizes ultrasound (US) to activate sonosensitizers to generate highly cytotoxic reactive oxide species (ROS), which has achieved great success in eradicating deep-seated bacterial infections. However, the limited penetration and low diffusion efficacy of sonosensitizers in biofilms severely impair the therapeutic effects of SDT. Herein, we design a near-infrared (NIR) light-driven nanomotor (MOF@Au-DNase I) with high electron–hole pairs separation efficiency for combating Staphylococcus aureus (S. aureus) biofilms. Specifically, gold nanoparticles (Au NPs) are deposited onto one-half of the sphere-shaped metal–organic framework (MOF) to construct Janus MOF@Au NPs, followed by the covalent immobilization of deoxyribonuclease (DNase I) onto the surface of MOF@Au. Under the NIR laser irradiation, MOF@Au NPs exhibit efficient active motion and penetrated quickly into deep biofilms within 15 min. Notably, DNase I can destroy the compactness of biofilms by hydrolyzing extracellular DNA (eDNA) in biofilms to facilitate the penetration of sonosensitizers. Moreover, the asymmetric spatial distribution of Au can significantly improve the electron transfer efficiency of MOF@Au and finally enhance the ROS generation. The synergistic effect enhances the efficiency of SDT-mediated therapy of the Janus MOF@Au NPs. The high ROS generation capacity of MOF@Au-DNase I realizes superior bactericidal effects in vitro and accelerates the healing process of biofilm-infected cutaneous wounds in vivo, demonstrating a flexible and effective strategy for biofilm eradication.

更新日期：2023-08-16

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