多组学揭示长双歧杆菌促进混合物种生物膜形成的机制,Food & Function

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多组学揭示长双歧杆菌促进混合物种生物膜形成的机制
Food & Function ( IF 5.1 ) Pub Date : 2023-08-14 , DOI: 10.1039/d3fo01751f
Tao Xu _{1,

2} , Yue Xiao _{1,

2} , Hongchao Wang _{1,

2} , Jinlin Zhu _{1,

2} , Wenwei Lu _{1,

2,

3,

4} , Wei Chen _{1,

2,

4}

Affiliation

前期研究发现长双歧杆菌、卵形拟杆菌、粪肠球菌和加氏乳杆菌在体外共培养时能更好地形成生物膜，而长双歧杆菌是该群落中促进生物膜形成的核心菌株。长双歧杆菌是肠道核心微生物群的一部分，被广泛认为是益生菌。因此，有必要通过转录组学和代谢组学探讨其在混合物种生物膜中的作用。代谢组学表明，氨基酸和嘌呤含量的增加可以促进生物膜的形成。转录组分析发现，许多与长双歧杆菌碳水化合物代谢、氨基酸代谢和环境耐受性相关的基因均上调。结合京都基因与基因组百科全书（KEGG）通路分析和基因本体（GO）分析，混种生物膜中长双歧杆菌的差异表达基因（DEG）主要与“群体感应（QS）”相关， “ABC转运蛋白”、“氨基酸的生物合成”、“不同环境下的微生物代谢”、“碳水化合物代谢”和“双组分系统”。此外，根据蛋白质-蛋白质相互作用（PPI）网络分析，在有机物代谢和氨基酸生物合成中发挥作用的rpl和rps基因家族是核心DEG 。最后，通过结合代谢组学和群体感应机制，发现自诱导肽（脯氨酰甘氨酸和甘氨酰亮氨酸）、N-酰基高丝氨酸内酯（N- (3-氧代羟基)高丝氨酸内酯）和AI-2的代谢可以促进生物膜的形成，包括由长双歧杆菌组成的单一物种和混合物种生物膜。我们的研究使我们能够了解长双歧杆菌在混合物种生物膜中的关键作用以及生物膜代谢与肠道健康之间的相互作用。此外，所产生的知识对于我们未来开发具有有益功能的生物膜产品具有重要意义。

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Multiomics reveals the mechanism of B. longum in promoting the formation of mixed-species biofilms

It has been found previously that Bifidobacterium longum, Bacteroides ovatus, Enterococcus faecalis, and Lactobacillus gasseri can form a biofilm better when co-cultured in vitro and B. longum is the core biofilm-formation-promoting strain in this community. B. longum is part of the core microbiota in the gut and is widely recognized as a probiotic. Therefore, it is necessary to explore its role in mixed-species biofilms through transcriptomics and metabolomics. Metabolomics showed that the increase in amino acid and purine content could promote biofilm formation. In transcriptomic analysis, many genes related to carbohydrate metabolism, amino acid metabolism, and environmental tolerance of B. longum were up-regulated. Combined with the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Ontology (GO) analysis, the differentially expressed genes (DEGs) of B. longum in mixed-species biofilms were mainly correlated to “quorum sensing (QS)”, “ABC transporters”, “biosynthesis of amino acids”, “microbial metabolism in different environments”, “carbohydrate metabolism” and “two-component system”. In addition, the rpl and rps gene families, which function in the metabolism of organic substances and the biosynthesis of amino acids, were the core DEGs according to the analysis of the protein–protein interaction (PPI) network. Finally, by combining metabolomics and quorum sensing mechanisms, it was found that the metabolism of autoinducer peptides (proliylglycine and glycylleucine), N-acyl homoserine lactone (N-(3-oxo hydroxy) homoserine lactone), and AI-2 can promote the formation of biofilms, both mono- and mixed-species biofilms composed of B. longum. Our research enabled us to understand the critical role of B. longum in mixed-species biofilms and the interactions between biofilm metabolism and gut health. In addition, the generated knowledge will be of great significance for us to develop biofilm products with beneficial functions in future.

更新日期：2023-08-14

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