Faster and more convenient construction of different functional multi-input–multi-output molecular logic devices has become a current research hotspot. In this study, a complex was synthesized using bovine serum albumin (BSA) and hydrazide dihydroxydisalicylaldehyde by a combinatorial approach. Then, the interaction mechanism was investigated by fluorescence spectroscopy and molecular simulation. Both methods revealed that the interaction between BSA and hydrazide dihydroxydisalicylaldehyde mainly occurred at site I of BSA. The main types of interactions included hydrogen bonding and van der Waals forces. Based on hybridized fluorescence resonance and electron exchange energy transfer, the complex generated two fluorescence emission peaks. Then, a digital comparator and a molecular logic circuit were designed using different metal ions or pH to modulate the output signals. Notably, the apparent increase in the fluorescence lifetime of the donor serves as an important basis for electron exchange between the donor and the acceptor.

更快、更便捷地构建不同功能的多输入多输出分子逻辑器件已成为当前的研究热点。在本研究中，使用牛血清白蛋白（BSA）和酰肼二羟基二水杨醛通过组合方法合成了复合物。然后，通过荧光光谱和分子模拟研究了相互作用机制。两种方法均表明BSA与酰肼二羟基二水杨醛之间的相互作用主要发生在BSA的I位点。相互作用的主要类型包括氢键和范德华力。基于杂交荧光共振和电子交换能量转移，该配合物产生了两个荧光发射峰。然后，设计了数字比较器和分子逻辑电路，使用不同的金属离子或pH值来调制输出信号。值得注意的是，供体荧光寿命的明显增加是供体和受体之间电子交换的重要基础。