卵转铁蛋白通过 MAPK 和 NF-κB 通路抑制 TNF-α 诱导的胃上皮细胞炎症反应,Journal of Agricultural and Food Chemistry

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卵转铁蛋白通过 MAPK 和 NF-κB 通路抑制 TNF-α 诱导的胃上皮细胞炎症反应
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-08-11 , DOI: 10.1021/acs.jafc.3c00950
Yan Huang _{1,

2,

3} , Shuping Chen _{1,

2,

3} , Yao Yao _{1,

2,

3} , Na Wu _{1,

2,

3} , Mingsheng Xu _{1,

2,

3} , Huaying Du _{1,

2,

3} , Zhongping Yin _{1,

2,

3} , Yan Zhao _{1,

2,

3} , Yonggang Tu _{1,

2,

3}

Affiliation

卵转铁蛋白（OVT）已被证实具有抗炎活性。然而，其对胃炎症的作用和机制尚不清楚。本研究探讨了OVT对肿瘤坏死因子-α（TNF-α）诱导的胃上皮细胞（GES-1）炎症反应的影响和机制。采用酶联免疫吸附试验（ELISA）测定炎症细胞因子的水平，然后通过RNA测序探索其抗炎作用的潜在途径，然后通过Western blotting和途径抑制剂进行验证。结果表明，浓度为 50–400 μg/mL 的 OVT 对 GES-1 细胞无毒性。此外，100 μg/mL的OVT明显减少白细胞介素（IL）-8、IL-6和TNF-α的分泌，分别降低63.02%（630.09/1703.98）、35.53%（935.81/1451.43）和36.19%（964.60）。 /1511.63），分别。RNA测序结果表明，OVT显着影响丝裂原激活蛋白激酶（MAPK）和激活B细胞核因子κ轻链增强子（NF-κB）通路的激活，并通过p-IKK、p-IκB、p-P65、p-ERK、p-JNK 和 p-P38 蛋白。GES-1细胞与NF-κB和MAPK通路抑制剂孵育后释放的IL-8含量进一步证实OVT阻碍了这两条通路的激活。总的来说，这些结果表明 OVT 是一种天然蛋白质，具有治疗胃部炎症的潜力。

"点击查看英文标题和摘要"

Ovotransferrin Inhibits TNF-α Induced Inflammatory Response in Gastric Epithelial Cells via MAPK and NF-κB Pathway

Ovotransferrin (OVT) has been confirmed to have anti-inflammatory activity. However, its effect and mechanism on gastric inflammation are unclear. In this study, the effect and mechanism of the OVT on the tumor necrosis factor-α (TNF-α) induced inflammatory response in gastric epithelial cells (GES-1) were investigated. The enzyme linked immunosorbent assay (ELISA) was used to determine the levels of inflammation cytokines, followed by RNA sequencing to explore the potential pathways of its anti-inflammatory effect, and then it was validated by Western blotting and pathways inhibitors. Results showed that the OVT at concentrations of 50–400 μg/mL displayed nontoxicity against GES-1 cells. Additionally, 100 μg/mL of OVT obviously reduced the secretion of interleukin (IL)-8, IL-6, and TNF-α by 63.02% (630.09/1703.98), 35.53% (935.81/1451.43), and 36.19% (964.60/1511.63), respectively. The results of RNA sequencing exhibited that the OVT significantly influences the activation of mitogen-activated protein kinase (MAPK) and the nuclear factor kappa-light-chain enhancer of activated B cell (NF-κB) pathways, which was verified by the levels of p-IKK, p-IκB, p-P65, p-ERK, p-JNK, and p-P38 protein. IL-8 contents released by GES-1 cells after incubation with inhibitors of NF-κB and MAPK pathways further confirmed that OVT hindered activation of these two pathways. Collectively, these results suggested that OVT was a natural protein with the potential to treat gastric inflammation.

更新日期：2023-08-11

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