当前位置：
X-MOL 学术
›
Food Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
MnO2 in-situ coated upconversion nanosystem for turn-on fluorescence detection of hypoxanthine in aquatic products
Food Chemistry ( IF 8.5 ) Pub Date : 2023-08-12 , DOI: 10.1016/j.foodchem.2023.137131 Yunrui Cao 1 , Yu Song 1 , Tingting Wei 1 , Tingyu Feng 1 , Meihuan Li 1 , Changhu Xue 2 , Jie Xu 1
Food Chemistry ( IF 8.5 ) Pub Date : 2023-08-12 , DOI: 10.1016/j.foodchem.2023.137131 Yunrui Cao 1 , Yu Song 1 , Tingting Wei 1 , Tingyu Feng 1 , Meihuan Li 1 , Changhu Xue 2 , Jie Xu 1
Affiliation
|
Hypoxanthine concentration is a potential indicator to evaluate the freshness in the early post-mortem of several aquatic products. Based on MnO in-situ coated upconversion nanoparticles (UCNPs) and xanthine oxidase (XOD), a novel sensor was conducted for the efficient, sensitive determination of hypoxanthine. In this strategy, upconversion fluorescence quenched by MnO would be restored by HO and uric acid (UA), two products from the XOD-catalyzed reactions of hypoxanthine. Through pretreatment with short-time heating and alkylation by -ethylmaleimide (NEM) to avoid potential interference from reducing substances in the food matrix, this method exhibited satisfactory selectivity. The fluorescence intensity of green emission I was positively proportional to hypoxanthine concentration at a wide range of 0.5–50 mg/L with a detection limit of 0.14 mg/L. Moreover, this convenient method was employed to quantify the hypoxanthine in fish, shrimp, and shellfish samples, showing excellent potential for the application in quality control of aquatic products.
中文翻译：
MnO2原位涂覆上转换纳米系统用于开启荧光检测水产品中的次黄嘌呤
次黄嘌呤浓度是评估几种水产品宰后早期新鲜度的潜在指标。基于MnO原位涂覆的上转换纳米粒子（UCNP）和黄嘌呤氧化酶（XOD），开发了一种新型传感器，用于高效、灵敏地测定次黄嘌呤。在该策略中，被MnO猝灭的上转换荧光将被H2O和尿酸（UA）恢复，这两种产物来自次黄嘌呤的XOD催化反应。通过短时间加热和-乙基马来酰亚胺（NEM）烷基化的预处理，以避免食品基质中还原物质的潜在干扰，该方法表现出令人满意的选择性。绿色发射 I 的荧光强度与次黄嘌呤浓度呈正比，范围为 0.5~50 mg/L，检测限为 0.14 mg/L。此外，这种简便的方法还用于定量鱼、虾和贝类样品中的次黄嘌呤，在水产品质量控制中显示出良好的应用潜力。
更新日期：2023-08-12
中文翻译：
MnO2原位涂覆上转换纳米系统用于开启荧光检测水产品中的次黄嘌呤
次黄嘌呤浓度是评估几种水产品宰后早期新鲜度的潜在指标。基于MnO原位涂覆的上转换纳米粒子（UCNP）和黄嘌呤氧化酶（XOD），开发了一种新型传感器，用于高效、灵敏地测定次黄嘌呤。在该策略中，被MnO猝灭的上转换荧光将被H2O和尿酸（UA）恢复，这两种产物来自次黄嘌呤的XOD催化反应。通过短时间加热和-乙基马来酰亚胺（NEM）烷基化的预处理，以避免食品基质中还原物质的潜在干扰，该方法表现出令人满意的选择性。绿色发射 I 的荧光强度与次黄嘌呤浓度呈正比，范围为 0.5~50 mg/L，检测限为 0.14 mg/L。此外，这种简便的方法还用于定量鱼、虾和贝类样品中的次黄嘌呤，在水产品质量控制中显示出良好的应用潜力。
京公网安备 11010802027423号