The level of butyrylcholinesterase (BChE) in living organisms is closely linked to disease occurrence. Developing effective methods to monitor BChE levels in living systems is essential for investigating the pathogenesis of BChE-related diseases and for early screening of the diseases. However, due to the lack of intuitive imaging tools, the expression level of BChE in many diseases remains to be explored. In this study, an ultrasensitive near-infrared fluorescent probe, LAN-bche, was developed to explore BChE expression in liver cancer, liver injury, and diabetes. LAN-bche is stable, water-soluble, ultrasensitive and high selective for BChE without disturbing from AChE, can detect BChE in dual-mode using fluorescence strategy (LOD = 0.056 U/L) and colorimetric strategy (LOD = 4.92 U/L). LAN-bche was employed to investigate the BChE expression both in liver cancer and liver injury diseases and confirmed that BChE expression was downregulated in both models, which was speculated to be due to the destruction of ability to generate BChE caused by the damage and cancerization of the liver cells. Furthermore, BChE expression in diabetes was explored and confirmed the upregulation of BChE expression in diabetes. In brief, our study shed light on BChE expression in liver cancer, liver injury, and diabetes and provides support for further research on the pathogenesis of BChE-related diseases. Besides, the probe LAN-bche is a robust tool for tracking BChE that is poised to facilitate imaging studies of BChE expression in numerous disease models.

中文翻译：

---

使用有前景的荧光成像工具探索疾病中丁酰胆碱酯酶的表达


生物体中丁酰胆碱酯酶（BChE）的水平与疾病的发生密切相关。开发有效的方法来监测生命系统中的 BChE 水平对于研究 BChE 相关疾病的发病机制和早期筛查疾病至关重要。然而，由于缺乏直观的成像工具，BChE在许多疾病中的表达水平仍有待探索。在这项研究中，开发了一种超灵敏的近红外荧光探针 LAN-bche，用于探索肝癌、肝损伤和糖尿病中 BChE 的表达。 LAN-bche 稳定、水溶性、超灵敏、对 BChE 具有高选择性，不受 AChE 干扰，可使用荧光策略 (LOD = 0.056 U/L) 和比色策略 (LOD = 4.92 U/L) 双模式检测 BChE 。 LAN-bche用于研究肝癌和肝损伤疾病中BChE的表达，证实两种模型中BChE表达均下调，推测这是由于肝癌和肝损伤疾病导致BChE产生能力被破坏所致。肝细胞。此外，对糖尿病中BChE表达进行了探索并证实了糖尿病中BChE表达的上调。简而言之，我们的研究揭示了BChE在肝癌、肝损伤和糖尿病中的表达，并为进一步研究BChE相关疾病的发病机制提供了支持。此外，探针 LAN-bche 是追踪 BChE 的强大工具，有望促进多种疾病模型中 BChE 表达的成像研究。