远 UVC 和 UVB 引起的 DNA 损伤取决于皮肤类型,Experimental Dermatology

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远 UVC 和 UVB 引起的 DNA 损伤取决于皮肤类型
Experimental Dermatology ( IF 3.5 ) Pub Date : 2023-08-07 , DOI: 10.1111/exd.14902
Loris Busch _{1,

2} , Marius Kröger ₁ , Daniela F Zamudio Díaz _{1,

3} , Johannes Schleusener ₁ , Silke B Lohan ₁ , Jackie Ma ₄ , Christian Witzel ₅ , Cornelia M Keck ₂ , Martina C Meinke ₁

Affiliation

波长 222 nm 和 233 nm 的远 UVC 辐射源由于其高皮肤相容性而成为皮肤消毒处理的一种有趣的潜在替代方案。然而，迄今为止，尚未发表有关远紫外线对不同皮肤类型引起的 DNA 损伤的研究，而这正是本研究的目的。用波长 222 和 233 nm 的远 UVC 以及宽带 UVB 照射皮肤后，使用免疫组织化学筛选组织中的环丁烷嘧啶二聚体阳性 (CPD + )^细胞。233 nm 照射后，深色皮肤类型的表皮 DNA 损伤低于白皙皮肤类型。与此相反，222 nm 的照射不会引起皮肤类型依赖性差异，这可归因于辐射穿透深度的降低。^{当使用 3 mJ/cm 2}的红斑下剂量时，UVB 在浅色和深色皮肤类型之间表现出相对最强的差异。由于黑色素以其光保护作用而闻名，我们使用双光子激发荧光寿命成像 (TPE-FLIM) 评估了不同皮肤类型样本中基底层和颗粒层中黑色素含量的比率，发现不同皮肤类型的比率较高四至五。由于已知远 UVC 只渗透到活皮肤的上层，因此上述黑色素比率可以解释与 UVB 相比，远 UVC 照射后皮肤类型之间差异不太明显的原因。

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Far-UVC- and UVB-induced DNA damage depending on skin type

Far-UVC radiation sources of wavelengths 222 nm and 233 nm represent an interesting potential alternative for the antiseptic treatment of the skin due to their high skin compatibility. Nevertheless, no studies on far-UVC-induced DNA damage in different skin types have been published to date, which this study aims for. After irradiating the skin with far-UVC of the wavelengths 222 and 233 nm as well as broadband UVB, the tissue was screened for cyclobutane pyrimidine dimer-positive (CPD⁺) cells using immunohistochemistry. The epidermal DNA damage was lower in dark skin types than in fair skin types after irradiation at 233 nm. Contrary to this, irradiation at 222 nm caused no skin type-dependent differences, which can be attributed to the decreased penetration depth of radiation. UVB showed the relatively strongest differences between light and dark skin types when using a suberythemal dose of 3 mJ/cm². As melanin is known for its photoprotective effect, we evaluated the ratio of melanin content in the stratum basale and stratum granulosum in samples of different skin types using two-photon excited fluorescence lifetime imaging (TPE-FLIM) finding a higher ratio up to skin type IV–V. As far-UVC is known to penetrate only into the upper layers of the viable skin, the aforementioned melanin ratio could explain the less pronounced differences between skin types after irradiation with far-UVC compared to UVB.

更新日期：2023-08-07

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