The root extract of Peucedanum ostruthium (PO-E) was identified as a promising antibacterial source from a screening of 158 extracts against Staphylococcus aureus. It has also recently been shown to significantly decrease the survival of the nematode Caenorhabditis elegans. We used the biochemometric approach ELINA to investigate the phytochemical characteristics of the multicomponent mixture PO-E to identify the anti-infective constituent(s) targeting S. aureus and C. elegans. ¹H NMR spectra of PO-E-derived microfractions were correlated with their respective bioactivity data. Heterocovariance analyses unambiguously identified ostruthin as an anti-staphylococcal constituent, which potently also inhibited Enterococcus spp.. ELINA demonstrated that anthelmintic activity was due to a combinatorial effect of ostruthin and isoimperatorin. A C. elegans-based survival and motility assay confirmed that isoimperatorin, imperatorin, and verapamil modulated the susceptibility of ostruthin. The combinatorial effect of these natural products was shown in larvae studies to be related to the function of the nematodes’ efflux pump.

通过对 158 种针对金黄色葡萄球菌的提取物进行筛选，发现前胡根提取物(PO-E) 是一种有前景的抗菌来源。最近还被证明可以显着降低线虫秀丽隐杆线虫的存活率。我们使用生物化学计量学方法 ELINA 研究多组分混合物 PO-E 的植物化学特征，以确定针对金黄色葡萄球菌和秀丽隐杆线虫的抗感染成分。^{PO-E 衍生的微级分的1} H NMR 谱与其各自的生物活性数据相关。异协方差分析明确确定了 ostruthin 是一种抗葡萄球菌成分，也能有效抑制肠球菌属。 ELINA 证明，驱虫活性是由于 ostruthin 和异欧前胡素的组合作用。基于线虫的存活和运动测定证实异欧前胡素、欧前胡素和维拉帕米调节ostruthin的敏感性。幼虫研究表明，这些天然产物的组合效应与线虫外排泵的功能有关。