In this study, the active components of the plant were carefully extracted and identified using three solvent systems. After extraction, we used solvent systems to further purify the main flavonoid chemical constituent. As a result of our analytical strategy, which included HPLC analysis, MS/MS spectroscopic analysis, and NMR data-based constructions, quercetin was determined to be the main chemical constituent. Our study suggests the potential therapeutic advantages of quercetin, a compound found in the leaves of Acalypha indica, for treating breast cancer cell lines MCF-7 and MDA-MB-231. Our comparison of Quercetin to the regularly prescribed medicine Doxorubicin shows that it has the capacity to inhibit MCF-7 and MDA-MB-231 cells. Measurements of apoptosis and cell cycle phase showed this to be the case. Furthermore, a ladder that formed as a result of cellular damage brought on by ROS provided further proof of the drug's impact on DNA integrity. Notably, pro-apoptotic proteins displayed increased apoptosis activity in cells treated with quercetin. Given that it is extracted from plants and has less adverse effects than other compounds, quercetin is a viable option for further clinical study. The objective is to fight breast cancer, one of the most prevalent diseases in the world and a main cause of death for women. Thus, our research makes a significant addition to the ongoing search for potent, plant-based breast cancer treatments.

在这项研究中，使用三种溶剂系统仔细提取和鉴定了植物的活性成分。提取后，我们使用溶剂系统进一步纯化主要黄酮化学成分。根据我们的分析策略（包括 HPLC 分析、MS/MS 光谱分析和基于 NMR 数据的构建），槲皮素被确定为主要化学成分。我们的研究表明槲皮素（一种在铁苋菜叶子中发现的化合物）对于治疗乳腺癌细胞系 MCF-7 和 MDA-MB-231 具有潜在的治疗优势。我们将槲皮素与常规处方药阿霉素进行比较，结果表明它具有抑制 MCF-7 和 MDA-MB-231 细胞的能力。细胞凋亡和细胞周期阶段的测量表明情况确实如此。此外，由于 ROS 引起的细胞损伤而形成的梯子进一步证明了该药物对 DNA 完整性的影响。值得注意的是，在用槲皮素处理的细胞中，促凋亡蛋白显示出增加的凋亡活性。鉴于槲皮素是从植物中提取的，并且比其他化合物具有更少的副作用，因此槲皮素是进一步临床研究的可行选择。其目标是对抗乳腺癌，乳腺癌是世界上最流行的疾病之一，也是女性死亡的主要原因。因此，我们的研究为持续寻找有效的植物性乳腺癌治疗方法做出了重大贡献。