Microchimica Acta ( IF 5.3 ) Pub Date : 2023-08-02 , DOI: 10.1007/s00604-023-05870-4 Xiaoxiao Hu 1 , Cuilu Quan 1 , Tiantian Ren 1 , Linan Zhao 1 , Yanting Shen 1 , Yanyan Zhu 1 , Jing Wang 1
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A novel stimulus-responsive surface-enhanced Raman scattering (SERS) nanoprobe has been developed for sensitive glutathione (GSH) detection based on manganese dioxide (MnO2) core and silver/gold nanoparticles (Ag/Au NPs). The MnO2 core is not only capable to act as a scaffold to amplify the SERS signal via producing “hot spots”, but also can be degraded in the presence of the target and thus greatly enhance the nanoprobe sensitivity for sensing of GSH. This approach enables a wide linear range from 1 to 100 µM with a 2.95 µM (3σ/m) detection limit. Moreover, the developed SERS nanoprobe represents great possibility in both sensitive detection of intracellular GSH and even can monitor the change of intracellular GSH level when the stimulant occurs. This sensing system not merely offers a novel strategy for sensitive sensing of GSH, but also provides a new avenue for other biomolecules detection.
Graphical Abstract
中文翻译：
饰有 Ag/Au 纳米标签的 MnO2 纳米粒子，用于基于标记的 SERS 测定细胞谷胱甘肽
基于二氧化锰 (MnO 2 ) 核心和银/金纳米颗粒 (Ag/Au NP)，开发了一种新型刺激响应表面增强拉曼散射 (SERS) 纳米探针，用于灵敏的谷胱甘肽 (GSH) 检测。MnO 2核心不仅能够充当支架通过产生“热点”来放大SERS信号，而且还可以在靶标存在的情况下被降解，从而大大提高纳米探针感测GSH的灵敏度。这种方法可实现 1 至 100 µM 的宽线性范围，检测限为 2.95 µM (3σ/m)。此外，所开发的SERS纳米探针不仅可以灵敏地检测细胞内GSH，甚至可以监测刺激物发生时细胞内GSH水平的变化。该传感系统不仅为GSH的灵敏传感提供了一种新颖的策略，而且还为其他生物分子的检测提供了新的途径。
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