Fragment Screening and Fast Micromolar Detection on a Benchtop NMR Spectrometer Boosted by Photoinduced Hyperpolarization,Angewandte Chemie International Edition

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Fragment Screening and Fast Micromolar Detection on a Benchtop NMR Spectrometer Boosted by Photoinduced Hyperpolarization
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2023-07-31 , DOI: 10.1002/anie.202308692
Gabriela R Stadler ₁ , Takuya F Segawa ₂ , Matthias Bütikofer ₁ , Venita Decker ₃ , Sandra Loss ₄ , Barbara Czarniecki ₄ , Felix Torres _{1,

5} , Roland Riek ₁

Affiliation

Benchtop NMR spectrometers are compact, lower in cost, and operate cryogen-free. The presented method using photoinduced hyperpolarization allows the detection of a protein-fragment interaction by a reduction of the ligand signal, simply by comparing the spectra measured in absence and presence of protein. Our approach enables to screen at micromolar concentrations of ligand and low micromolar concentrations of protein on a benchtop NMR.

中文翻译：

光诱导超极化增强的台式 NMR 波谱仪上的片段筛选和快速微摩尔检测

台式核磁共振波谱仪结构紧凑，成本较低，并且无需制冷剂即可运行。所提出的使用光诱导超极化的方法允许通过减少配体信号来检测蛋白质片段相互作用，只需比较在不存在和存在蛋白质的情况下测量的光谱。我们的方法能够在台式 NMR 上筛选微摩尔浓度的配体和低微摩尔浓度的蛋白质。

更新日期：2023-07-31

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